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Ultrasonic-assisted Cellulase Extraction of Polysaccharides from Sparassis crispa |
FENG Liguo1,CHEN Xianglian2,XU Ning1,HUANG Xiaohui1* |
1Edible Fungus Institute of Hunan Province,Changsha 410013,Hunan,China;2Hunan College of Medicine,Huaihua 418000,Hunan,China |
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Abstract Ultrasound-assisted cellulase was used to extract polysaccharides from Sparassis crispa,optimize the experimental design and determine the best extraction conditions.This paper conducted a singlefactor experiment on 5 factors,including ultrasonic power,ultrasonic time,ultrasonic temperature,material-to-liquid ratio,and amount of cellulase added.It was confirmed that under the conditions of 570 W ultrasonic power and 3% cellulase added amount,the material-to-liquid ratio,ultrasonic time,and ultrasonic temperature were used as 3 interactive factors to carry out Box-Behnen experimental design and response surface optimization.The best solution was as following:the material-to-liquid ratio was 1∶87.62(g · mL-1),ultrasonic temperature was 38.4 ℃,and ultrasonic time was 94.56 min,then the predicted polysaccharide yield could reach 31.49%. After verifying the best forecast model paramter,the results showed that under the conditions of material-to-liquid ratio 1∶90 (g · mL-1),extraction temperature 38 ℃,extraction time 95 min,the extraction rate of crude polysaccharide from 5 g Sparassis crispa powder could reached as high as 30.6%.The crude polysaccharide extraction was also performed on 50 g Sparassis crispa powder.The first extraction rate was 31.70%,and the second extraction rate was 7.1%.After concentrating the extracted crude polysaccharide,deproteinizing,depigmenting,and purifying by dialysis,the polysaccharide retention rate was 73.20%.
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Received: 07 September 2020
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