Abstract: Excessive application of nitrogen fertilizer will reduce crop quality and harm environment．
How to reduce the nitrogen fertilizer applying dosage without reduction of output has become an issue urgently
to be solved for agriculture development．Arbuscular mycorrhizae fungi（AMF） plays a positive role in
promoting plant nutrient absorption，growth and development，and yet has the potential of replacing nitrogen
fertilizer．Taking lettuce（Lactuca sativa L. var. ramose Hort.） and spinach（ Spinacia oleracea L.） as research
objects，this experiment set up 8 treatments with different nitrogen fertilizer dosage and 5 treatments with
different AMF use level，respectively．This experiment screened out the best dosages of pure nitrogen fertilizer
for lettuce and spinach growth being 6.0 kg · hm^-2 and 8.0 kg · hm^-2，respectively and the best use level of
AMF were 200 g · kg^-1 and 175 g · kg^-1，respectively．Then AMF was used to replace the nitrogen fertilizer in
different proportions．Afterwards，the paper studied the effect of AMF on reducing nitrogen fertilizer dosage
in lettuce and spinach．The results showed that under the condition of substrate cultivation and on the premise
of not reducing lettuce and spinach fresh weight，adding 200 g · kg^-1 and 175 g · kg^-1 AMF，respectively could
reduce 40% and 60% nitrogen fertilizer dosage in lettuce and spinach production，respectively．That is，the
nitrogen fertilizer dosages were 3.6 kg · hm^-2 for lettuce and 3.2 kg · hm^-2 for spinach，their plant growth were
unaffected．The experiment result provided a new way for reducing nitrogen fertilizer application in lettuce and
spinach and for high-quality cultivation as well．

Key words: arbuscular mycorrhizal fungi, nitrogen fertilizer reduction, lettuce, spinach, substrate cultivation

摘要： 过量施用氮肥会降低作物品质且危害环境，如何在不减产的情况下降低氮肥施用量成为农业发展中亟待解决的问
题。丛枝菌根真菌（arbuscular mycorrhizae fungi，AMF）在促进植物营养吸收及生长发育方面发挥积极作用，具有替代氮肥
的应用潜力。本试验以叶用莴苣（Lactuca sativa L. var. ramose Hort.）与菠菜（Spinacia oleracea L.）为研究对象，分别设置8
个不同氮肥用量处理与5 个不同AMF 菌剂用量处理，筛选出叶用莴苣和菠菜生长最好的纯氮用量分别为6.0 kg · hm^-2 和8.0
kg · hm^-2，AMF 用量分别为200 g · kg^-1 和175 g · kg^-1。用AMF 替代不同比例的氮肥用量，研究AMF 对叶用莴苣和菠菜氮
肥用量的减施效果。结果表明，基质栽培条件下，在不降低全株鲜质量的前提下，基质中分别添加200 g · kg^-1 和175 g · kg^-1
AMF 可减少叶用莴苣40% 和菠菜60% 的氮肥用量，即叶用莴苣和菠菜氮肥用量分别为3.6 kg · hm^-2 和3.2 kg · hm^-2 时，植
株生长不受影响。试验结果为叶用莴苣和菠菜氮肥减施和高品质栽培提供了新途径。

关键词: 丛枝菌根真菌, 氮肥减施, 叶用莴苣, 菠菜, 基质栽培