|
|
|
| Optimization of High Efficient Tissue Culture Regeneration System for Ornamental and Edible Dual Purpose Lily—‘ Jinghe’ |
| QI Xianyu1 ☆，ZHANG Wenliang1，2 ☆，YANG Panpan1，XU Leifeng1*，MING Jun1* |
| 1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2College of Biological Science and Technology，Shenyang Agricultural University，Shenyang 110866，Liaoning，China |
|
|
|
|
Abstract Lily is an important high-grade vegetable and ornamental flower．It is based on scale high efficient tissue culture regeneration system which is the foundation for large-scale production and reproduction of lily bulbs．This experiment used glyphosate for the first time as differentiation-inducing agent to optimize tissue culture regeneration system taking scale as explant of lily variety ‘Jinghe’ with ornamental and edible dualpurpose．The result indicated that the optimal induction medium for‘Jinghe’scale adventitious bud was MS +1.0 mg · L-1 6-BA + 0.1 mg · L-1 NAA + 2.5 mg · L-1 glyphosate + 30 g · L-1 saccharose，and the induction coefficient was 6.46，which was significantly higher than that of the conventional induction medium．The optimal subculture medium was MS + 0.5 mg · L-1 6-BA + 0.1 mg · L-1 NAA + 30 g · L-1 saccharose，and the proliferation coefficient was 4.01．The best medium for bulb expansion was MS + 90 g · L-1 saccharose．The best rooting medium was 1/2MS + 0.3 mg · L-1 NAA + 30 g · L-1 saccharose．The rooting coefficient was 23.59．
|
|
Received: 10 December 2021
Published: 10 June 2022
|
|
|
|
|
|
|
