Abstract: QuEChERS method was adopted to conduct sample pretreatment．Through optimizing chromatography and mass spectrometric condition，a QuEChERS method combined with ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）was established to rapidly test the residues of 5 pesticides and their metabolites in cowpea samples．This method was used to detect 238 commercially available cowpea samples in Hainan Province from November 2020 to February 2021．The health risks of these pesticide residues were also evaluated．The results showed that the quantification limits of aldicarb and its metabolites was 0.010 mg · kg^-1．The quantification limits of the rest 4 pesticides and their metabolites was 0.005 mg · kg^-1．Two pesticides and one metabolite were detected from samples．Detection rate of cyromazine was 44.5%．That of carbendazim was 22.3%．That of melamine was 0.8%．Dietary intake risk assessment was conducted on these 2 detected pesticides．The result showed that the risk of acute dietary intake of pesticides was 0.375 8%-29.126 6%，and risk of chronic dietary intake of pesticides was 0.057 6%-0.131 4%．Their value-at-risk were far less than 100%，indicating their risks were within the accepted range．It means that probability of threatening dietary safety of consumer through pesticide residues intake from cowpea was lower．

Key words: cowpea, pesticide, metabolite, ultra performance liquid chromatography-tandem mass spectrometry（UPLC-MS/MS）, dietary intake risk assessment

摘要： 采用QuEChERS技术进行样品前处理，通过优化色谱-质谱条件，建立了豇豆中5种农药及其代谢物快速检测的方法—QuEChERS法结合超高效液相色谱-串联质谱法，采用该方法对海南省2020年11月至2021年2月采集的238份市售豇豆样品进行检测，并对农药残留的健康风险进行评估。结果表明，涕灭威及其代谢物的定量限为0.010 mg·kg^-1，其他4种农药及其代谢物的定量限为0.005 mg·kg^-1；样品中共检出2种农药及1种代谢物：灭蝇胺的检出率为44.5%，多菌灵的检出率为22.3%，灭蝇胺代谢物三聚氰胺的检出率为0.8%。对检出的2种农药进行膳食摄入风险评估：急性膳食摄入风险范围为0.3758%～29.1266%，慢性膳食摄入风险范围为0.0576%～0.1314%，风险值远小于100%，均处于风险可接受范围内，表明通过豇豆摄入的农药残留量对消费者的膳食安全构成威胁的概率较低。

关键词: 豇豆, 农药, 代谢物, 超高效液相色谱-串联质谱, 膳食摄入风险评估