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| Creation of Cabbage Interspecific Material with Clubroot Resistance and Ogura CMS Restorer |
| YANG Ding1，2，LYU Fengxian1，2，LI Chongjuan1，2，XU Xuezhong2，3，HU Jingfeng2，3，YANG Hongli2，3，LAN Mei2，3，ZHANG Liqin2，3，DONG Xiangshu1，HE Jiangming2，3* |
| （1Yunnan University，Kunming 650504，Yunnan，China；2Horticultural Crops Research Institute，Yunnan Academy of Agricultural Sciences，Kunming 650205，Yunnan，China；3Yunnan Branch of National Vegetable Improvement Center，Kunming 650205，Yunnan，China） |
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Abstract In order to obtain cabbage Ogura CMS interspecific material resistant to clubroot disease and carrying restorer gene，this experiment took cabbage‘Xiangan 366’resistant to clubroot as female parent and Ogura CMS restorer material Brassica napus L.‘N21015’as male parent；carried out distant hybridization combined with embryo rescue．Then the interspecific hybrids were obtained．The results of clubroot resistance phenotypic identification and molecular marker identification showed that hybrid F1 was immune to clubroot disease，and had the maternal clubroot resistance gene and paternal Ogura CMS restorer gene．After artificially induced chromosome doubling，heterohexaploid interspecific hybrids were successfully obtained，and the pollen fertility rate was 78.46%．
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Received: 16 May 2022
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