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| Selective Breeding of Sweet Pepper Genic Male Sterile Line Dual Purpose System Assisted by KASP Molecular Marker |
| FAN Yanqin，YAN Libin，MENG Yaning，ZHANG Hongxiao |
| Institute of Economic Crops，Hebei Academy of Agriculture and Forestry Sciences，Shijiazhuang 050051，Hebei，China |
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Abstract In order to further improve the breeding efficiency of genic male sterile line dual purpose system of sweet pepper，KASP molecular markers were designed by using the mutation site of the‘ AB91’genic male sterile candidate gene Capana05g000747．The F2 population and dual purpose system of transformation sweet pepper genic male sterile lines were used as test materials to detect the genotype of plant fertility and check the accuracy of this marker．The results indicated that the molecular marker presented common separation with the fertility of genic male sterile sweet pepper．Early identification was carried out on genotype of F2 plant．The classification accuracy were 98.91% and 99.04%，respectively．The effective eliminated genotype was the fertile plant of MSMS，which could be directly used for transformation of sterile line．The dual purpose system of yellow and red genic male sterile lines sweet peper‘AB91-HTJ412’and‘AB91-HLD163’were turn out．During seedling period，early stage identification on fertility genotypes of dual purpose system population could effectively eliminate the fertile plants of MSms genotype．And the selected and remaining genotype was sterile plants of msms，which could be directly used in cross combination breeding and hybrid seed production．
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Received: 01 June 2022
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