Abstract:

In order to alleviate the damage of high temperature stress on the seedlings of protected cucumber，the cucumber‘Jinchun No.4’was used as the test material，and the putrescine（Put），melatonin（MT），proline（Pro）and potassium fulvic acid（MFA）was mixed at different concentrations to investigate the effects of different concentration mixtures on high temperature resistance，photosynthesis，and antioxidant enzyme activity of cucumber seedlings，and screened out the optimal mixture for alleviating high temperature stress．The results showed that 8 mmol·L^-1 Put combined with 0.3 g·L^-1 MFA，1.5 mmol·L^-1 Pro and 50 μmol·L^-1 MT effectively alleviated the damage of high temperature stress on cucumber seedlings and the effect was the best comprehensively evaluated with the membership function method．The net photosynthetic rate increased by 160.71%，the leaf relative electrolyte leakage，malondialdehyde and H₂O₂ content decreased by 47.26%，68.00% and 40.64%，respectively．Furthermore，SOD，POD and CAT activities in cucumber leaves increased by 29.32%，31.66% and 52.16%，respectively．

Key words: cucumber, high temperature, putrescine, melatonin, potassium fulvic acid, proline

摘要： 为缓解高温胁迫对设施黄瓜幼苗的伤害，以津春4号为试材，将腐胺（Put）、褪黑素（MT）、脯氨酸（Pro）和黄腐酸钾（MFA）以不同的浓度进行复配，研究不同浓度组合对黄瓜幼苗高温抗性、光合作用和抗氧化酶活性等的影响，筛选出缓解高温胁迫伤害最优的配方。结果表明：8 mmol·L^-1 Put配合施用0.3 g·L^-1 MFA、1.5 mmol·L^-1 Pro及50 μmol·L^-1 MT能够有效缓解高温胁迫对黄瓜幼苗的伤害，采用隶属函数法综合评价效果最佳，与对照相比净光合速率增加了160.71%，H₂O₂含量降低了40.64%，可溶性蛋白含量增加了126.92%，SOD、POD、CAT活性分别提高了29.32%、31.66%、52.16%。

关键词: 黄瓜, 高温, 腐胺, 褪黑素, 黄腐酸钾, 脯氨酸