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Isolation and Identification of Fusarium oxysporum in Protected Tomato at Shouguang City and Studies on Tomato Varieties Susceptibility to Disease |
WU Yafen,XIANG Dan,LI Lin,LIANG Bin* |
(College of Resources and Environment,Qingdao Agricultural University,Qingdao 266109,Shandong,China) |
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Abstract A total of 18 pathogenic strains(SG1-SG18)were isolated and purified from tomato plants infected by Fusarium wilt from greenhouses in Shouguang City of Shandong Province.Koch’s Postulates was used to verify the pathogenicity of the strain.Furthermore,the strains were identified by morphological and molecular biological identification methods.The results showed that the isolated and purified pathogenic strains were Fusarium oxysporum f. sp. lycopersici.The disease was tomato Fusarium wilt.Among all strains,SG6,SG7 and SG17 were physiological race 1 of tomato Fusarium wilt,and the rest were physiological race 3 of tomato Fusarium wilt.Results of pot experiment by inoculating 3 physiological varieties of tomato fusarium wilt to different tomato varieties showed that‘Busan 88’was a relatively typical susceptible variety of 3 physiological races of tomato Fusarium wilt.While‘Saint Anne’and‘Strawberry tomato’were more resistant varieties to Fusarium wilt suitable for cultivation in Shouguang area.
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Received: 26 July 2022
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