China Vegetables

Previous Articles     Next Articles

Comprehensive Evaluation on Fruit Quality and Disease Resistance of Pepper Germplasm Resources

LI Zixiong,PAN Bingqing,SONG Yingying,MA Shijie,ZHANG Di,CHEN Jie,SHEN Huolin*,SUN Liang*   

  1. College of Horticulture,China Agricultural University,Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops,Beijing 100193,China
  • Received:2023-03-27 Online:2023-05-16 Published:2023-08-01

辣椒种质资源果实品质与抗病性综合评价

李子雄 ,潘兵青,宋莹莹, 马世杰, 张 娣 ,陈 婕 ,沈火林* ,孙 亮*   

  1. 中国农业大学园艺学院,设施蔬菜生长发育调控北京市重点实验室,北京 100193
  • 通讯作者: 孙亮,男,副教授,博士生导师,专业方向:蔬菜种质创新与分子设计育种,E-mail:liang_sun@cau.edu.cn; 沈火林,男,教授,博士生导师,专业方向:蔬菜种质创新与分子设计育种,E-mail:shl1606@cau.edu.cn
  • 作者简介:李子雄,硕士,专业方向:蔬菜种质创新与分子设计育种,E-mail:lzxyxa@163.com;潘兵青,博士研究生,专业方向:蔬菜种质创新与分子设计育种,E-mail:panbingqing2022@163.com
  • 基金资助:
    三亚崖州湾科技城项目(SYND-2002-25,SYND-2021-19),海南省重大科技计划项目(ZDKJ2021010),科技创新服务能力建设- 高精尖学科建设(CEFF-PXM2019_014207_000032)

Abstract: In order to screen out high-quality and multi-resistant pepper germplasms,this paper took 109 domestic and foreign pepper germplasms as test material;measured 6 traits of fruit quality;and identified whether they carried TMV resistant gene L4,Phytophthora capsici resistant gene CaPhyto,TSWV resistant gene Tsw and PVY resistant gene Pvr4. Then,the paper carried out comprehensive evaluation through genetic diversity analysis,correlation analysis,principal component analysis,cluster analysis and aggregated boosted tree(ABT)analysis. Results showed that the genetic diversities of 109 pepper germplasms were abundant,and coefficient of variation ranged from 24.51%-123.52%. Among them β-carotene content showed maximum variation degree. ABT analysis also indicated that capsanthin had the greatest influence on indexes determined by colorimeter,followed by lutein and that of β-carotene was minimum. The resistance molecular markers had identified 8 accessions carrying L4 gene,23 carrying CaPhyto gene and 1 carrying Pvr4 gene. The principal component analysis and cluster analysis showed that 109 pepper germplasms could be divided into 3 groups.The quality indexes of group I and group II were all relatively excellent. Finally,9 germplasms with both good quality and resistance were screened out.

Key words: pepper, germplasm resources, fruit quality, disease resistance, cluster analysis, aggregated boosted tree analysis

摘要: 为筛选出优质多抗辣椒种质资源,以109 份国内外辣椒种质资源为试材,对6 个果实品质性状进行了测定,同时对是否携带抗烟草花叶病毒病基因L4、抗辣椒疫病基因CaPhyto、抗番茄斑点萎蔫病毒病基因Tsw 和抗马铃薯Y 病毒病基因Pvr4 进行了鉴定。通过遗传多样性分析、相关性分析、主成分分析、聚类分析和聚合推进树(ABT)分析进行了综合评价。结果表明:109 份辣椒种质资源遗传多样性丰富,变异系数介于24.51%~123.52% 之间,其中β- 胡萝卜素含量变异程度最大。ABT 分析表明,辣椒红素对色差仪测定各指标影响最大,叶黄素次之,而β- 胡萝卜素最小。抗性分子标记鉴定筛选出8 份材料携带L4 基因,23 份材料携带CaPhyto 基因,1 份材料携带Pvr4 基因。主成分分析和聚类分析表明,109 份辣椒种质资源可分为3 个类群,类群Ⅰ和类群Ⅱ的品质指标均较为优异。最终,筛选出9 份兼具品质与抗性的优异种质。

关键词: 辣椒, 种质资源, 果实品质, 抗病性, 聚类分析, 聚合推进树分析