Abstract: This study aimed to identify strains of the tomato spotted wilt virus（TSWV）infecting peppers in Guizhou，and establish rapid detection techniques，specifically TSWV loop-mediated isothermal amplification（LAMP）．We collected Twelve pepper samples suspected of TSWV infection and ten samples of Western flower thrips from major pepper-producing regions in Guizhou Province．These samples were analyzed
using RT-PCR，LAMP detection techniques and hylogenetic tree construction based on TSWV-N genes．Molecular diagnosis and sequencing results confirmed the presence of TSWV in the suspected pepper samples．Additionally，he pathogenic isolates of TSWV in peppers from the Xifeng area of Guizhou exhibited a striking 99% similarity to Guizhou tomato TSWV-N isolates．Building upon these findings，a TSWV rapid detection
system for pepper crops and the vector Thrips occidentalis was successfully established，providing strong support for the monitoring and control of pepper virus diseases in Guizhou．

Key words: tomato spotted wilt virus, western flower thrips, LAMP detection, molecular diagnosis

摘要： 为明确侵染贵州辣椒的番茄斑萎病毒（Tomato spotted wilt virus，TSWV）株系，并建立TSWV 环介导等温扩增（LAMP）等快速检测技术，从贵州省辣椒主产区采集疑似感染TSWV 的辣椒样本12 份和西花蓟马样本10 份，并应用RT-PCR、LAMP 检测技术，以及基于TSWV-N 基因系统发育树构建进行研究。通过分子诊断和测序结果分析，确认疑似感染辣椒样本被TSWV 侵染。此外，贵州息烽地区辣椒TSWV 病毒病原分离物与贵州番茄TSWV-N 分离物相似度最高，为99%。基于这些发现，成功建立了针对辣椒作物和传播媒介西花蓟马的TSWV 快速检测系统，为贵州辣椒病毒病害的监测和防控提供有力支持。

关键词: 番茄斑萎病毒, 西花蓟马, LAMP 检测, 分子诊断