In Vitro Culture and Regeneration System Establishment of Pepper

LI Haoyu1; 2; JIA Li2; SONG Tingting2; WANG Sheng1; 2; ZHANG Yu1; 2; FANG Ling2; ZHANG Qi’an2; YAN Congsheng2; SUI Yihu1*; JIANG Haikun2*

doi:10.19928/j.cnki.1000-6346.2023.2048

China Vegetables ›› 2024, Vol. 1 ›› Issue (1): 38-45.DOI: 10.19928/j.cnki.1000-6346.2023.2048

In Vitro Culture and Regeneration System Establishment of Pepper

LI Haoyu1，2，JIA Li2，SONG Tingting2，WANG Sheng1，2，ZHANG Yu1，2，FANG Ling2，ZHANG Qi’an2，YAN Congsheng2，SUI Yihu1*，JIANG Haikun2*

1Anhui Science and Technology University，Fengyang 233100，Anhui，China；2Institute of Horticulture，Anhui Academy of Agricultural Sciences，Horticultural Crop Germplasm Creation and Physiological Ecology Key Laboratory of Anhui Province，Key Laboratory for Germplasm Creation and Utilization of Horticultural Crops，Ministty of Agriculture and Rural Affairs（Ministry-Province Joint Construction），Hefei 230031，Anhui，China

Received:2023-07-27 Revised:2023-09-22 Online:2024-01-10 Published:2024-01-01

辣椒离体培养及再生体系的建立

李浩宇1，2，贾利2，宋婷婷2，汪胜1，2，张于1，2，方凌2，张其安2，严从生2，隋益虎1*，江海坤2*

1 安徽科技学院，安徽凤阳233100；2 安徽省农业科学院园艺研究所，园艺作物种质创制及生理生态安徽省重点实验室，农业农村部园艺作物种质创制与利用重点实验室（部省共建），安徽合肥230031

通讯作者: 隋益虎，男，教授，硕士生导师，专业方向：蔬菜遗传育种，E-mail：suiyihu2004@126.com；江海坤，男，研究员，硕士生导师，专业方向：蔬菜遗传育种，E-mail：jhk211@163.com
作者简介:李浩宇，男，硕士研究生，专业方向：蔬菜遗传育种与分子生物学，E-mail：2859788403@qq.com
基金资助:
财政部和农业农村部：国家现代农业产业技术体系项目（CARS-23-G40），安徽省科技重大专项（202203a06020030），安徽省蔬菜产业技术体系专项经费资助项目（皖农科函[2021]711 号），安徽省农业科学院青年英才经费资助项目（QNYC-202121）

Abstract

Abstract: In order to establish an efficient in vitro regeneration system for chili peppers，five different genotypes of aseptic chili peppers were selected，and the effects of the addition of MS medium with different hormone ratios on bud induction and differentiation，elongation and proliferation，root growth and transplantation were investigated．The results showed that the combination of MS + 6.0 mol·L-1 6-BA + 1.0 mol·L-1 IAA + 5.0 mol·L-1 AgNO3 was the optimal medium for bud differentiation，which could induce white fluffy healing tissues，with a 100.0% induction rate of Flamingo-bill and 86.7% of cotyledons．MS + 3.0 mol·L-1 6-BA + 2.0 mol·L-1 IAA + 5.0 mol·L-1 AgNO3 + 1.5 mol·L-1 GA3 was the optimal medium for adventitious bud elongation，with 66.7% ratios of adventitious bud elongation in Flamingo-bill and 30.0% ratios in cotyledons adventitious bud elongation．MS + 0.5 mol·L-1 NAA was the optimal medium for adventitious bud rooting，the rooting rate of both Flamingo-bill and cotyledon adventitious bud was 100.0%．Regenerated plants were obtained from both Flamingo-bill and cotyledon explants.

Key words: pepper, in vitro culture, plant regeneration, explant

摘要： 为了建立高效的辣椒离体再生体系，选择5种不同基因型的辣椒无菌苗，研究了添加不同激素配比的MS培养基对芽诱导分化、芽伸长增殖、生根和移栽等的影响。结果表明：MS + 6.0 mol·L-1 6-BA + 1.0 mol·L-1 IAA + 5.0 mol·L-1 AgNO3组合为芽分化的最适培养基，可诱导出白色松软的愈伤组织，Flamingo-bill 诱导率为100.0%，子叶诱导率为86.7%。MS + 3.0 mol·L-1 6-BA + 2.0 mol·L-1 IAA + 5.0 mol·L-1 AgNO3 + 1.5 mol·L-1 GA3 组合为不定芽伸长的最适培养基，Flamingo-bill不定芽伸长率为66.7%，子叶不定芽伸长率为30.0%。MS + 0.5 mol·L-1 NAA 组合为不定芽生根的最适培养基，Flamingo-bill和子叶不定芽生根率均为100.0%。通过Flamingo-bill和子叶外植体均能获得再生植株。

关键词: 辣椒, 离体培养, 植株再生, 外植体

LI Haoyu1，2，JIA Li2，SONG Tingting2，WANG Sheng1，2，ZHANG Yu1，2，FANG Ling2，ZHANG Qi’an2，YAN Congsheng2，SUI Yihu1*，JIANG Haikun2*. In Vitro Culture and Regeneration System Establishment of Pepper[J]. China Vegetables, 2024, 1(1): 38-45.

李浩宇1，2，贾利2，宋婷婷2，汪胜1，2，张于1，2，方凌2，张其安2，严从生2，隋益虎1*，江海坤2*. 辣椒离体培养及再生体系的建立[J]. 中国蔬菜, 2024, 1(1): 38-45.

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In Vitro Culture and Regeneration System Establishment of Pepper

辣椒离体培养及再生体系的建立

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