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| Induction and Purification of β-Glucanase from Trichoderma harzianum and Its Growth Promoting and Disease Prevention Effects on Cucumber Seedlings |
| YANG Chunlin1，2 ，LI Honghao1 ，HU Qiang3 ，XI Yadong1* |
| 1 The Institute of Plant Protection，Sichuan Academy of Agricultural Sciences，Vegetable Germplasm Innovation and Variety Improvement Key Laboratory of Sichuan Province，Chengdu 610066，Sichuan，China；2 Leshan Plant Protection and Quarantine Station of Sichuan Province，Leshan 614000，Sichuan，China；3 Bamboo Diseases and Pests Control and Resources Development Key Laboratory of Sichuan Province，Leshan Normal University，Leshan 614000，Sichuan，China |
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Abstract Under laboratory condition，this experiment took β-glucan，wheat bran and cell wall of
Fusarium oxysporum as the only carbon source to induce Trichoderma harzianum Th-30 fermentation and β-glucanase production，and isolate and purify β-glucanase by ammonium sulfate salting out and agarose gel column chromatography．Taking ‘Jinyan 4’ cucumber as test material，this experiment also explored the effects of β-glucanase on physiological and morphological indexes of resistance，and prevention and control function of cucumber seedling on common soil-borne diseases of cucumber．The results showed that the activities of β-glucanase from Trichoderma Th-30 fermentation induced under different conditions were significantly different．The enzyme activity of crude enzyme liquid induced by β-glucan was the highest，reaching 75.63 U · mL-1 at 72 h of fermentation．The specific activity of purified β-glucanase was 783.56 U · mg-1 ，being 45.32 times of the crude enzyme．The treatments of 5-fold solution and 10-fold solution of β-glucanase could increase the root activity and free proline content，PAL，POD，and PPO activities of cucumber seedling，as well as the root shoot ratio and healthy seedling strength index，and the control effects of 10-fold solution of β-glucanase on cucumber Rhizoctonia root rot，cucumber Fusarium wilt，cucumber blight，cucumber damping-off，cucumber stem rot were 50.36%-80.63%．
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Received: 12 August 2023
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