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| Root Phenotype Analysis on Pumpkin Seedling Used as Rootstock and Evaluation about Its Tolerance to Hypertonic Stress |
| （1National Nanfan Institute，Chinese Academy of Agriculture Sciences，Sanya 572024，Hainan，China；2State Key Laboratory of Vegetable Bio-breeding，Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China） |
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Abstract Abstract：In order to investigate the correlation between root morphology index of pumpkin seedling used as rootstock，total sugar content and its tolerance to hypertonic stress，and also the development situation of pith cavity under hypertonic stress，this study took 16 pumpkin varieties used as rootstock as test material； measured the root system phenotype parameters of the seedlings 14 d after sowing by root scanning method； and extracted root configuration；determined content of total sugar in root system；screened varieties with greater differences in root characters through principal component analysis（PCA）and one-way ANOVA，etc.；and also measured the growth indexes of stems，leaves and root system，contents of total sugar and development situation of pith cavity under hypertonic stress．Then，the tolerance of pumpkin seedlings used as rootstock to hypertonic stress were evaluated comprehensively．The results showed that the seedling root system phenotypic parameters of 16 varieties after dimension reduction showed greater differences between Blackseed Pumpkin and Kato Aoki．Nine root phenotypic indexes including total root length，root surface area and root branching numbers，etc. and root total sugar content of Blackseed Pumpkin were significantly higher than those of Kato Aoki．After hypertonic stress treatment，the stem，leaf and root growth indexes of Blackseed Pumpkin were significantly superior to those of Kato Aoki．Furthermore，the root system total sugar content of Blackseed Pumpkin still remained relatively stable even after 15 d persistent hypertonic stress treatment. Besides，the hypocotyl pith cavity development was less sensitive to hypertonic stress．In conclusion，the main morphological parameters and total sugar content in root system can be used as important reference indexes for screening rootstock resistant to hypertonic stress．The Blackseed Pumpkin has the highest overall ranking and shown a stronger tolerance to hypertonic stress．It is an excellent rootstock adaptable to certain hypertonic stress environment．
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Received: 10 October 2023
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