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| Identifi cation and Stress Response Analysis of Potato CDF Transcription Factors |
| CHANG Jing1，WANG Zemin2，3*，HU Yunjie3，HE Wenjing3，JIN Xin2，3，HOU Lele3，LI Xuan3，AI Qianyi3 |
| 1Gansu Provincial Seed Station，Lanzhou 730000，Gansu，China；2State Key Laboratory of Aridland Crop Science，Gansu Agricultural University，Lanzhou 730070，Gansu，China；3College of Life Science and Technology，Gansu Agricultural University，Lanzhou 730070，Gansu，China |
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Abstract To explore the role of the StCDFs transcription factor family in potato stress response，bioinformatics methods were employed to analyze the phylogenetic relationship，gene structure，conserved motifs，cis-acting elements，tissue-specific expression，hormone and stress response of StCDFs．Simultaneously，transcriptome data and qRT-PCR were used to analyze the transcription levels of StCDFs after diff erent cold-tolerant varieties were treated with low temperature stress．A total of 5 StCDFs members（StCDF1，StCDF2，StCDF3，StCDF4，StCDF5）were identified in potato．The CDF family of potato is closely related to tomato．The analysis of gene structure and conserved Motif revealed that Motif 1 was the most widely distributed and conserved in the potato StCDF．Cis-acting element analysis showed that the upstream promoter of StCDFs gene contained a wide range of hormone and stress response elements，and stress response elements accounted for the largest proportion．Tissue-specifi c expression analysis revealed that StCDFs exhibited distinct expression patterns in diff erent tissues．Additionally，most of these genes were found to respond to stress treatment．Based on the RNA-seq data，the StCDFs were found to respond positively to low temperature stress treatment．Subsequently，the qRT-PCR was performed to explore the expression levels of StCDFs and found that the expression levels of StCDF1，StCDF2 and StCDF3 were up-regulated under low temperature stress．
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Received: 25 May 2024
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