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| Effi cient Regeneration System for Brassica olerecea Based on Epicotyl Explants |
| HAN Xichao1，2，ZHANG Xiaojian2，CAI Xu2，LIANG Jianli2，WANG Xiaowu2，ZHU Keming1*，WU Jian2* |
| 1College of Life Sciences，Jiangsu University，Zhenjiang 212013，Jiangsu，China；2Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，State Key Laboratory of Vegetable Biobreeding，Beijing 100081，China |
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Abstract The hypocotyl is a commonly used explant for genetic transformation of cabbage（Brassica oleracea var. capitata），however，the problems of genotype-dependent and low regeneration efficiency are the main obstacles at present．This study explores the construction of a genetic transformation system using the epicotyl of cabbag e as an explant，with 9 cabbage varieties as test materials．The regeneration effi ciency after Agrobacterium infection was compared between the hypocotyl and epicotyl as two explants．The results show that 5 cabbage varieties had elongated epicotyls．After Agrobacterium inoculation，all 5 varieties with epicotyls as explant induced adventitious shoots with regeneration frequencies ranging from 32.4% to 78.4%．In contrast，only 3 varieties induced of adventitious buds with hypocotyl as explant，with the highest regeneration frequency of only 2.7%．This shows that the epicotyl is an ideal explant for overcoming genotypic dependence in B. olerecea transformation，and provides an effi cient approach to obtain regenerated B. oleracea plants．
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Received: 09 April 2024
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