Artificial freezing identification, clustering analysis combined with natural frosting identification in field were adopted to review the freezing tolerance and type of freezing tolerance of clone material from S.acaule offspring. Among 25 progenies of S.acaule, before cold acclimation, 11 were sensitive to frost and freezing, 12 were tolerant and 2 were strong tolerant. But after cold acclimation, the progenies sensitive to the freezing declined to 3, while the tolerant and strong tolerant increased to 12 and 10, respectively. The LT50 of the experimental genotypes before and after cold acclimation, the cold aclimation ability were extremely significantly related to the field freezing rating score. The correlation coefficient were 0.50, 0.91 and -0.55, respectively. Therefore, it is concluded that the artificial freezing results could be used to forecast the freeze tolerance in field in potato production.