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Studies on the Effective Factors of Microspore Culture in Chinese Cabbage |
Han Yang; Ye Xueling2; Feng Hui |
Department of Life Sciences; Liaoning University; Shenyang 110036; College of Horticulture; Shenyang Agricultural University; Shenyang 110161 |
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Abstract The experiment on culture technique of the isolated microspore was conducted with Futian 50 and Changkuai genotypes of Chinese cabbage as the materials.The results showed that 0.1-1.0 mg·L -1 NAA inhibited the embryo emergence.2,4-D strongly inhibited the formation of microspore embryo.Low density of ctytokinin improved the frequency of microspore embryogenesis.The optimal content of ZT was 0.2 mg·L -1 and 6-BA was 0.4 mg·L -1 .The influence of 6-BA is greater than ZT.Comprehensive influence of auxin and ct...
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Received: 12 October 2005
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