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| Studies on Relation between DNA Methylation and Ploidy of Plant Regeneration from Microspore of Pakchoi |
| YAO Jun1,2,WU Jian2,WANG Xiao-wu2,QIN Zhi-wei1* |
| 1Horticulture College,Northeast Agricultural University,Harbin 150030,Heilongjiang,China;2Institute of Vegetables
and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China |
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Abstract By isolated microspore culture technique from pakchoi(Brassica rapa ssp.chinensis),9 regeneration plants
including 2 haploids and 7 diploids were obtained.The spontaneously double rate of diploid was up to 77.7 %.Two
haploids and 2 diploids were selected to construct their respective DNA pool.Combining MethylationSensitive
Restriction Endonuclease-PCR method with SRAP marker technique,it gained 8 primer combinations showing polymorphism
from 768 pairs of primers.It indicated that the ploidy level of regeneration plants from pakchoi may be related to
DNA methylation in plant genomics.
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Received: 26 February 2009
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