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Construction and Transformation of Inverted Repeat of Turnip Mosaic Virus Coat Protein Gene |
Luo Jing~ 1; 2; Chen Wei~ 1; Zhuang Mu~ 2; et al. |
~ 1 College of Life Science; Capital Normal University; Beijing 100037; ~ 2 Insititute of Vegetables and Flowers; Chinese Academy of Agricultural Sciences; Beijing 100081 |
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Abstract The construct using inverted repeat sequences of the coat protein gene of TuMV was designed,and introduced into Sijiucaixin via Agrobacterium tumefaciens-mediated transformation system.13 positive transformants were obtained through PCR analysis.The transgenic efficiency was up to 2 %.
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Received: 23 October 2006
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