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| Separation and Identification of Bacillus LBC-1 against Alternaria brassicae |
| LI Chun-hui1，LI Shu-na1，WANG Na-xian1，DU Shu-tao1，WANG Shi-ying1，ZHU Bao-cheng1 |
| 1College of Life Science,Hebei University,Baoding 071002,Hebei,China;2College of Life Science,Hebei Agricultural University,Baoding 071001,Hebei,China |
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Abstract Twenty strains of Bacillus were isolated by sampling soil from fields of various regions via preliminary screening.They have antagonistic activities against Alternaria brassicae.A strain named LBC-1 with a relatively higher antagonistic activity was obtained via secondary screening.Strain LBC-1 was characterized by physiological and biochemical experiments,morphological features observation,and 16S rDNA sequence analysis.Based on the results,strain LBC-1 was finally identified as Bacillus amyloliquefaciens.Strain LBC-1 was a broad-spectrum antagonistic strains of spore-forming bacteria.
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Received: 26 February 2009
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