ISSN 1000-6346
CN 11-2326/S
中华人民共和国农业农村部 主管
中国农业科学院蔬菜花卉研究所 主办
CN 11-2326/S
中华人民共和国农业农村部 主管
中国农业科学院蔬菜花卉研究所 主办
Effects of different washing treatments on tomato rotten rates within 7 days
shelf life,packed by PE film at ambient temperature(28-30 ℃),were investigated,including
tap water,100 mg·kg-1 NaClO and 5 mg·kg-1 ClO2 under different temperature(5 ℃ higher and
lower than fruits’ temperature,respectively).The results showed that the rotten rates of
the washed tomatoes were decreased significantly from 47 %(no washed,CK)to 9 %-26 %,and the
temperature has not taken effect on reducing rotten rate for tap water and ClO2
treatments.However,it reduced the rotten rate significantly by 100 mg·kg-1 NaClO washing
treatment with 5 ℃ higher than fruits temperature ompared with 5 ℃ lower than fruits’
temperature treatment,which reached 9 %,the best treatment after 7 days storage.
It is a comparison about the chemical composition and nutritional components of
the fruit and leaf of genuine traditional Lycium barbarum L. ‘Ningqi No.1’produced from
Luhuatai Horticultural Farm of Yinchuan and Zhongning County.The results show that the
highest amino acids contents are Asp,Glu,Ala,Arg and Pro both in fruits and leaves,but no
significant difference was observed between the fruits produced in Luhuatai Horticultural
Farm of Yinchuan and Zhongning County.The lycine content of Air-drying fruit produced in
Zhongning County was 44.2 % higher than that produced in Luhuatai Horticultural Farm of
Yinchuan.The polysaccharide content of air-drying fruit and leaf produced in Luhuatai
Horticultural Farm of Yinchuan was higher than that produced in Zhongning County.The VC
content of air-drying fruit and leaf produced from Luhuatai Horticultural Farm of Yinchuan
was distinctively higher than that produced in Zhongning County.The loss of carotene
content of freeze-drying fruit was higher than that of air-drying fruit.While the loss of
VC content of air-drying fruit was higher than that of freeze-drying fruit.
Effect of molybdenum and manganese on nitrate reductase activity,nitrate content
and nutrient quality in Brassica campestris ssp.chinensis were studied using solution
culture by spraying Mo and Mn elements on leaf,root and leaf combined with root
treatment.The results indicated that nitrate reductase activity was significantly improved
by Mo treatment,which caused significant reduction of nitrate content.Although,no
remarkable effect on improving nitrate reductase activity was found by Mn treatment,nitrate
content was also reduced.Both Mo and Mn could improve the contents of soluble sugar and
VC.Compared with single treatment of Mo or Mn,the reduction of nitrate content,improvement
of soluble sugar and VC contents were more distinct by treatment with Mo plus Mn.
In this study,it established and optimized SSR detection system including
reaction components,amplified program,by using cabbage inbred lines 15R（‘Nanbao’）and
14S(‘Beiheidapingtou’).A stable SSR-PCR system was followed:10.00 μL reaction solution
contained 1×Buffer，50.00 μmol·L-1 dNTPs，0.40 U Taq enzymes，0.40 μmol·L-1 SSR primers
pairs，1.00 μL DNA(60.00 ng·μL-1).PCR amplifying program was divided into two steps,after
one denaturing step of 5 min at 94 ℃,DNA was denatured for 45 s at 94 ℃,the annealing
temperature was 60 ℃ and dropped 0.5 ℃ for each cycle,until a final annealing temperature
of 50 ℃ was reached.For the last 15 cycles of the amplification,an annealing temperature
of 55 ℃ was employed.When all cycles were finished,amplification ended for 10 min at 72 ℃
.The polyacrylamide gel electrophoresis was used in the detection of PCR amplification.
Taking different disease resistance pepper varieties(lines)as material,this paper
studies on the artificial inoculation as identification method of Leveillula taurica to
pepper from inoculation concentration,investigation time,age of inoculated seedling,disease
occurring condition after inoculation plant inoculation treatment method,etc.The results
showed that the most effective artificial inoculation method was inoculating at 15-leaf
stage and sprayed by a suspension of 1×104 conidia per mL and kept in dark and under 100 %
humidity during first 24 h after inoculation and then in 23-27 ℃ and 60 %-85 % humidity in
daytime,16-18 ℃ and 95 %-100 % humidity at night.
276 isolates were obtained from rhizospheric soils of pepper.It was found that 7
potential biocontrol actinomycetes showed good antagonistic effects on Phytophthora capsici
by pairing culture.The inhibition zone diameter ranged from 7.8 mm to 13.0 mm.The result
from the experiment trial at greenhouse showed that CQ21-3 strain could effectively control
the pepper phytophthora blight,the control efficiency reached 73.2 %.The result from
culture filtrate showed that CQ21-3 isolate was effective for inhibiting mycelium growth
and reducing spore germination of P.capsici in certain of concentration.
Forty strains of symbiotic bacteria were isolated from entomopathogenic
nematodes.Bioassay results showed that all strains had virulent activity to the first
instar larvae of Laphygma exigua and Colletotrichum gloeosporiodies to some extent,among
which Xenorhabdus nematophila A24-1 strain was the best one.The average corrected
mortalities to Laphygma exigua was 100.00 %,and the diameter of antibiotic circle to
Colletotrichum gloeosporiodies was 40.00 mm.
By applying the method of water culture with 1/2 Hoagland solution,this paper
studied the autotoxicity of pepper plants’ fermentation broths and allelopathy regulation
on it by gingko leaves’ fermentation broths and decomposition solutions that the
fermentation broths were decomposed again after the leaves were removed.The results showed
that under the autotoxicity of pepper plants’ fermentation broths,their root vigors were
declined and the relative conductivity was aggrandized.Gingko leaves’ fermentation broths
enhanced the autotoxicity of pepper plants’ fermentation broths,and showed positive
correlation with its concentration.Gingko leaves’ decomposition solutions have alleviated
the autotoxicity of pepper plants’ fermentation broths.The alleviated effects depend on
the concentration of gingko leaves’ decomposition solution.
A preliminary study was made on the allelopathy of liquid extract of Allium
ascalonicum L.to Lactuca sativa L.,Brassica chinensis L.,Raphanus sativus L.and Cucumis
sativus L.The results showed that the extract from Allium ascalonicum L.had some inhibit
effect on the germination of seed of Lactuca sativa L.and Raphanus sativus L.,had little
inhibit effect on the germination of seed of Brassica chinensis L.and Cucumis sativus L.The
results showed some stimulating effect on the growth of Lactuca sativa L.,Brassica
chinensis L.,Raphanus sativus L.and Cucumis sativus L.,as a whole.
Alternaria capsici-annui was studied in this article,which caused one of the main
decay of postharvest pepper.Oligochitosan and salicylie acid were used on pepper storage
and their effects to spore of A.capsici-annui were analyzed.The results showed that
Oligochitosan and salicylie acid could have significant effect on reducing black spot
incidence of post-harvest pepper,and the optimum concentration was 75 mg·L-1.
The multiple traits of mini watermelon can be reflected by several specific factors.The test divided the
traits into three types which composed sets and subsets of estimation elements.11 mini watermelon materials were
analyzed by comprehensive blur evaluation.These 3 characters double-fuzzy comprehensive evaluation results:
‘Linglongwang’general ranking was the first,Hai 024×Hai 011 F1 was the second,and Hai 024×Hai 011 F1 can be used
as a similar species of‘Linglongwang’got further comparative experiment.
The flower bud differentiation process of Xinjiang Artemisia dracunculus L. lasted for about one month from
late May to July.The process could be divided into three phases.They are floral axis differentiation
phase,inflorescence differentiation phase and flower organ differentiation phase.The apical flower bud and laterval
flower bud of tarragon can turn from vegetative cone and half flourish is imperfect flower and tublar floret is
perfect flower.
