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Current Issue
2013 Vol.1 Issue.10,
Published 2013-05-03
1
Studies on Applying
Solanum torvum
Sw. in Eggplant Graft
LI Nan-Yang, CHEN Yu-Hui, LIU Fu-Zhong, ZHANG Ying, LIAN Yong
Solanum torvum
Sw. which is relatived wild eggplant with good disease resistance and stress tolerance,is widely used as rootstock in Solanaceae crops cultivation.It has disease resistance and can increase production.The article reviews the research progress in applying
S. torvum
as rootstock in eggplant production,and studying the mechanism of resisting disease and overcoming stress injury by graft cultivation.
2013 Vol. 1 (10): 1-8 [
Abstract
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1357
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943KB] (
3687
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9
Research Progress in Seed Treatment
Mao-Lian-Gang, YAN Dong-Dong, WU Zhuan-Fang, MA Tao-Tao, WANG Qiu-Xia, LI Yuan, GUO Mei-Xia, OuYang-Can-Bin , CAO Ao-Cheng, ZHENG Jian-Qiu
Seed treatment is an economical and effective method in plant disease control.It adopts biological,physical,chemical technology to protect seed and plant,control the diseases and pests,insure the normal growth of crops,and achieve superior quality and high yield.According to the development history of seed treatment technology and differences between various seed treatment technology,this paper reviews the concepts,principles,classification,characteristics and application,so as to provide scientific guidance for seed treat ment in actual production.
2013 Vol. 1 (10): 9-15 [
Abstract
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825
) [
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1KB] [
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885KB] (
1415
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16
Impact of External Shock on Fluctuation of Vegetable Price —Based on Inter-provincial Panel Data VAR Model
LUO Chao-Ping, LI Wei-Yi, Zhai Qiong
On the basis of building panel vector auto-regression model(PVAR),the authors empirically analyzed the impact of external shock on fluctuation of vegetable price;and found that except GDP growth rate,which had a significant impact on price fluctuation,other variables contributed only 1.5%-3.3% to price variation. Meanwhile,considering the impact from variable,when given the subsidies-LBT a standard differential impact,the vegetable price-LJG would response negatively and reach a maximum in the third period.When given the vegetable disaster area LCZ a standard deviation, the vegetable price - LJG would initially turn a negative response then to positive response in the third period. When given GDP growth a deviation shock,vegetable price-LJG showed a distinct positive response.When given the LCZSR a deviation shock,vegetable price-LJG showed negative response. Therefore,the effect of external shock to vegetable price is not ideal,there exists hysteresis qualityin its response to partial external shock. Consequently,building a sound vegetable industry market mechanism,improving the efficiency of vegetable circulation,and ensuring the balance between vegetable supply and demand are important measures to inhibit t he violent fluctuation of vegetable price.
2013 Vol. 1 (10): 16-22 [
Abstract
] (
740
) [
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1KB] [
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943KB] (
878
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23
Distribution of S-haplotypes in Cabbage F
1
Hybrid
MIAO Wen-Wen, TIAN Lei, ZHUANG Mu, LIU Yu-Mei, YANG Li-Mei, ZHANG Yang-Yong, FANG Zhi-Yuan
DNA fragments corresponding to kinase domain of S locus kinase(
SRK
) gene were amplified by PCR with 3 pairs of specific primers in all of the 23 cabbage(
Brassica oleracea
L. var.
capitata
L.) F
1
hybrids.The S haplotypes of cabbage F
1
hybrid were preliminary identified via DNA sequencing and Blast analysis.The results indicated that 11 S haplotypes were found in cabbage F
1
hybrid,including class Ⅰ haplotypes,S7,S16,S28,S35,S45,S57,S68 and an unknown one named Sn,and class Ⅱ haplotypes S2,S5 and S15.The highest frequency of S haplotyes was S5 with 19.6% and followed by S28 with 17.4% in this study.Compared over 50 S haplotypes in
Brassica
,the 11 S haplotypes in cabbage F
1
hybrid suggests that it is very necessary for exploiting more S haplotypes in cabbage breeding programs.
2013 Vol. 1 (10): 23-28 [
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747
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1221KB] (
877
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29
Construction and Expression of a Cucumber Lipoxygenase Gene
CsLOX2
in Prokaryotic Cells
CHEN Qiao, CHEN Shu-Xia, WANG Cong-Ying, HAO Li-Ning, WAN Xu-Hua, SHEN Xiao-Qing, CHENG Zhi-Hui
The ORF of cucumber(Cucumis sativus L.)
CsLOX2
gene was inserted into an expression vector pGEX-4T-1 through PCR method using specific primers Yh1 and Yh2,and the recombined expression vector p4t-LOX2 was constructed. The expression amount of fusion protein was detected under different expressive conditions,such as different time,different temperature and different concentration of IPTG. The 115 kD fusion protein was obtained and induced by IPTG and it showed that
CsLOX2
gene encoded a 89 kD protein after the 26 kD GST protein tag was removed.The proper induction condition was 37 ℃ temperature,0.80 mmol·L
-1
IPTG concentration and 10.5 h inducing time.
2013 Vol. 1 (10): 29-35 [
Abstract
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817
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1KB] [
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1368KB] (
882
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36
Preliminary Study on Cold-induced Transcription Factor
CBF3
Transformed in Eggplant
MENG Ping-Hong, WAN Fa-Xiang, WANG Yong-Qing, WEN Lin-Hong, GUO Jing-Tao, SU Cheng-Gang
We successfully constructed cold-induced transcription factor
CBF3
(CRT binding factor),which was transferred to eggplant‘ Sanyueqie’(
Solanum melongena
L.)by agrobacteriummediated transformation. And we obtained 2 transgenic eggplant plants containing cold-tolerant gene
CBF3
,through Polymerase Chain Reaction(PCR)detection genomic DNA,and kanamycin solution daubing on the leaves of generation plants in the field confirmed that the transgenic plants of eggplant were positive.Thus,we successfully established regeneration and transformation systems in eggplant.
2013 Vol. 1 (10): 36-43 [
Abstract
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764
) [
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2236KB] (
877
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44
Initial Studies on Defense Gene Expression Mechanism of
Phaseolus vulgaris
L. var.
chinensis
Hort. to Anthracnose at Seedling Stage
HU Guo-Fu, XIAO Zi-Heng, ZENG Leng-Nan, ZHANG Ting, LI Cheng-Yan
Use susceptible cultivar‘Laolaishao’and resistant cultivar‘Dayoudou’as test materials,the relative expression of 5 defense enzyme genes(chitinase, β-1,3-glucanase,SOD, POD and LOX gene)and HRGP protein gene were detected and analyzed by real-time PCR after 2 cultivars infected by anthracnose in different times( 0,3,6,9,12 d). The results showed that in addition to the β-1,3- glucanase gene and SOD gene,the other 4 genes expression were higher in resistant varieties than the susceptible varieties before infected;after infected chitinase gene and the LOX gene expression showed decreased-increased trend in susceptible cultivar,and resistant varieties reached the peak,when susceptible varieties decreased to the minimum(3 d),the relative expression of resistant varieties was higher than that of the susceptible ones in the whole stage(0-12 d);β-1, 3-glucanase gene,POD gene and SOD gene were in the same trend,but the expression of resistant varieties was higher than the susceptible varieties.
2013 Vol. 1 (10): 44-49 [
Abstract
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767
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1KB] [
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1626KB] (
884
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50
Evaluation about Chlorpyrifos Residue in Fruit of Cucumber Germplasm Resources
YANG Jing, QIN Zhi-Wei, ZHOU Xiu-Yan, XIN Ming
In this experiment,38 cucumber(
Cucumis s
ativus L.) samples of different ecological types were tested.The chlorpyrifos pesticide was sprayed on cucumber samples during the full fruit period.Then we tested the samples with gas chromatography,in order to evaluate the chlorpyrifos residue in the main cucumber germplasm resources.The results showed that there were great differences between different samples fruits in the level of chlorpyrifos residue. The chlorpyrifos residue is 0.795 mg·kg
-1
on average,the change is between 0.072-1.835 mg·kg
-1
,and the CV is 0.549. Furthermore,different chlorpyrifos residue levels exists in cucumbers fruits of different ecological types and cucumbers from different producing areas. Only one sample,with chlorpyrifos residue lower than the national standard 0.1 mg·kg
-1
(GB2763—2005),was selected.
2013 Vol. 1 (10): 50-55 [
Abstract
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716
) [
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1KB] [
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991KB] (
661
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56
Effects of Different Bio-organic Fertilizer Application Rates on Controlling Bitter Gourd Fusarium Wilt,Soil Microorganisms and Enzyme Activities
REN Shuang, LIU Ying, CAO Qun, DING Wen-Juan, HE Yuan-Huan, GONG Qi-Xin, LI Hua-Xing-*
The pot experiment was conducted by using the bio-organic fertilizer(BOF)made by mixing the mature compost with functional bacteria to study the effects of different applying quantity of bio-organic fertilizer on controlling bitter gourd(
Momordica charantia
L.) Fusarium wilt and on soil microorganisms and enzyme activities.The results indicated that the occurrence of bitter gourd Fusarium wilt was decreased significantly by applying BOF.The disease-control effects of the 3 BOF treatments reached 22.21%,38.91% and 44.45%,respectively,when the diseasecontrol effect of CF was 0. With the same effective nutrient,the plant stem diameter,leaf length and leaf width,and plant dry weight under BOF2 treatment were significantly higher than that of CF treatment,with remarkable growth stimulating effect.The number of bacterium and actinomycesin soil were increased by BOF,while the amount of fungi and
Fusarium oxysporum
in soil were reduced evidently.Application of BOF could increase the activities of soil urease,phosphatase and invertase,which were higher than those of CF treatment.But the change of catalase was uncertain. The results showed that BOF could improve soil microbial flora and enhance soil enzyme activity,improve soil environment condition,and prevent the occurrence of Fusarium wilt.
2013 Vol. 1 (10): 56-63 [
Abstract
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972
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999KB] (
780
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64
Effects of Mixed Corn Stalk Substrates on Growth and Photosynthesis of Eggplant Seedlings
SU Li-Ying, SONG Shu-Yao, ZHAO Chun-Bo, ZHANG Xue-Mei, ZHAO Jing, ZHANG Yue
Taking eggplant(
Solanum melongena
L.)cultivar‘Shenqie No.1’as experiment material,and traditional peat and vermiculite as contrast,this study mixed corn stalk substrate with different sizes zeolite in various rations as seedling substrates and studied the physical and chemical properties of this substrates and its applying effect on eggplant seedling cultivation.The results showed that adding zeolite to seedling substrate could increase the water holding porosity,and decrease in its pH value and electrical conductivity( EC).When zeolite addition reached 20%-40%,the physical and chemical properties of this substrate could meet the requirement of seedling culture and make the emergence rate,growth vigor,and quality of eggplant seedling superior than that of the contrast. The media( corn stalk and zeolite ratio is 70
V
∶30
V
,zeolite in 10 mesh) could improve the quality of eggplant seedling.Its total chlorophyll content and photosynthetic parameters of eggplant seedling leaf were the highest,and the strong seedling index was significantly higher than those of the control and other treatments.
2013 Vol. 1 (10): 64-70 [
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801
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947KB] (
760
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71
Effect of Different Wall Materials on Temperature Effect of Greenhouse
LIANG Nan, LU Zhi-Quan, JIANG Xin-Mei, YU Xi-Hong, WU Feng-Zhi
This research used -40℃ outdoor temperature as designing standards and red brick wall as the contrast to study the effects of different wall materials on temperature effect of greenhouse.The results showed that the greenhouse wall structure using 240 mm,20 eyelets perforated brick as interior wall,120 mm XPS as external wall and 25 mm cement mortar as outermost layer,had higher endothermic and exothermic capacity,and could raise the soil and air te mperatures effectively.
2013 Vol. 1 (10): 71-75 [
Abstract
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673
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1KB] [
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945KB] (
686
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76
Effect of Different Treatments on Seed Germination of Water Shield (
Brasenia schreberi
J. F. Gmel)
LI Shuang-Mei, KE Wei-Dong, ZHU Hong-Lian
Using water shield(
Brasenia schreberi
J.F.Gmel)newly collected seed as material, the paper studied on the effects of different treatments on the germination rate and energy.The results showed that 4 °C low temperature treatment had better effect on improving the germination rate of water shield seed.It could get better effect to break dormancy of water shield seeds with the treatment of 4 °C cold water storage,soaking with 500 mg·L
-1
GA
3
for 6 h and then in 40 °C water for 50 min.25 °C was the best germination temperature.Under the condition of 20 °C, the seed would take long germination cycle,and under the condition of 30 °C,the seed was easy to breed white rot around germination hole. Under partial lighting conditions,seed had a higher germination rate,and almost no germination under dark condition.
2013 Vol. 1 (10): 76-79 [
Abstract
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1019
) [
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1KB] [
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908KB] (
748
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80
Headspace Sampling GC-MS Analysis of Volatile Flavor Components in Garlic Scape
WANG Chang-Zhu, GAO Jing-Cao, MENG Huan-Wen
The volatile flavor components in garlic(
Allium sativum
L.) scape were analyzed by using headspace sampling combined with GC-MS method.The results showed that garlic scape contains 23 kinds of volatile components,including 15 kinds of sulfur-containing compounds with 99.4% re lative content.The 6 main components are diallyl disulfide( 66.52%),1,3-dithiane(15.44%),diallyl sulfide(7.15%),dimethyl disulfide(1.24%),diallyl sulfide(1.09%) and 2-allyl methyl sulfide (2.66%).Compared with steam distillation method,sulfur compounds detected by headspace sampling method were fewer in kinds, but their contents were relatively higher,and the 3 main types ingredients: sulfide,multi-dithiane and thiocyclohexylene could be detected.
2013 Vol. 1 (10): 80-83 [
Abstract
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945
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1KB] [
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996KB] (
896
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84
Molecular Detection of Pepper Viruses in Southern Vegetable Production Bases
YAO Yu-Rong, CHEN Guo-Hua, FENG Lan-Xiang, XIE Bing-Yan
Pepper virus disease is a main disease in the southern vegetable production base in Guangdong and Hainan Provinces.It is necessary to detect the kinds of pathogeny,so as to provide references for the prevention of virus diseases. In this study,we conducted molecular detection with RT-PCR method on the samples of pepper virus diseases or suspected virus diseases from these 2 provinces.The results showed that the detection rate of viruses was 55.67% in Guangdong Province. The main viruses were CMV(
Cucumber mosaic virus
) and PMMoV(
Pepper mild mottle virus
).Besides, there were a few PVY(
Potato virus Y
),PVX(
Potato virus X
),and TYLCV(
Tomato yellow leaf curl virus
) detected.In Hainan Province,virus detection rate was 44.67%.CMV and PMMoV were the major viruses.There was also a small quantity of PVY. In the fields,the complex infection of CMV and PMMoV was the crucial problem in pepper production in Guangdong and Hainan Provinces.
2013 Vol. 1 (10): 84-89 [
Abstract
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874
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1KB] [
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970KB] (
1318
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90
Physiological Race Identification of Powdery Mildew for Edible Seed Watermelon and Melon in Some Regions of Xinjiang
SU Rui, WANG Hui-Lin, GAO Qiang, JIA Song-Nan, ZHENG Jian
Ten pathogen collections of powdery mildew,which came from different grown regions of Southern and Northern Xinjiang,were detected by standard system for identification of powdery mildew. According to the internationally recognized melon differential standards for identifying physiological races of powdery mildew,we confirmed that the disease was caused by
Sphaerotheca fuliginea
. There were two races, race 1 and race 5.Race 1 was the dominant one in Northern Xinjiang, and race 5 was in 2 regions of Southern Xinjiang.
2013 Vol. 1 (10): 90-94 [
Abstract
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855
) [
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1KB] [
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875KB] (
591
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95
A New Cucumber F
1
Hybrid for Greenhouse —‘Zhongnong No. 31’
GU Xing-Fang, ZHANG Sheng-Ping, WANG Ye, MIAO Han, ZHENG Qi-Gong
‘Zhongnong No.31’ is a newly bred cucumber F1 hybrid using inbred line 09676 as maternal parent and 081006 as paternal parent.Its fruit is about 35 cm in length with short neck and shining dark green skin.There are many white spines and small tuberculates on its fruit skin.It has multi-resistances for downy mildew,powdery mildew and Fusarium wilt,et al.Moreover,it is tolerant to low temperature and weak light. It is of early maturity and can sustainably bear fruit.It can yield over 150 t·hm
-2
.It is suitable for greenhouse cultivation in northern China.
2013 Vol. 1 (10): 95-96 [
Abstract
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635
) [
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1KB] [
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838KB] (
638
)
97
A New Seedling-edible Chinese Cabbage F
1
Hybrid —‘Sushengkuailyu’
LUO Zhi-Min, ZHANG Bin, WEN Feng-Ying, LIU Xiao-Hui, ZHAO Bing, WANG Chao-Nan, HUANG Zhi-Yin, LI Mei
‘Sushengkuailyu’ is a new seedling-edible Chinese cabbage F
1
hybrid bred by crossing self-incompatible line P42 with excellent DH line Feng520.It is upright and compact with 30 cm in height.It has beautiful appearance with long obovate leaf,bright green and glossy leaves,flat foliar without hairs,with green and wide flat petiole.Its leaf margin has few blunt lobes,with soft waxy texture.It grows rapidly and can be harvested at 25 days when the number of blades is over 10 after sowing.Its yield can reach about 50.475 t·hm
-2
.It is highly resistant to soft rot and virus disease, and resistant to downy mildew.It is suitable to be planted all over China.
2013 Vol. 1 (10): 97-99 [
Abstract
] (
689
) [
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1KB] [
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2585KB] (
605
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100
A New Thick-skin Melon F
1
Hybrid —‘Yongtian No.7’
WANG Yu-Hong, ZANG Quan-Yu, MA erLei, DING Wei-Hong, HUANG Fu-Wei-Guo, HUANG Yun-Ping
‘Yongtian No.7’ is an F1 hybrid melon with crispy flesh and thick skin. It is bred by crossing inbred line YW06-1 as female parent with YW10-3 as male parent.The fruit is ellipse in shape.Its single weight is about 1.8 kg.The peel is white in color with dense net.Its flesh is orange in color.It tastes crispy and fine.The Brix in the central part of fruit is over 15%.The fruit developing period is about 40 days in spring and 38 days in summer.The whole developing period is about 100- 110 days in spring and 80 days in summer.Field investigation shows that‘ Yongtian No. 7’ is resistant to gummy stem blight,and it can yield 16.2-36.0 t·hm
-2
.It is suitable for facility cultivation in spring and fall in eastern China region.
2013 Vol. 1 (10): 100-102 [
Abstract
] (
693
) [
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1KB] [
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921KB] (
597
)
103
A New Bitter Gourd F
1
Hybrid —‘Jiamei’
GAO Shan, LIN Bi-Ying, XU Duan-Xiang, LIN Feng
‘Jiamei’is a new bitter gourd F1 hybrid developed by crossing predominant female line 43-C and inbred line 59-W.The plant grows vigorously. The first female flower is set at 8th-12th nodes. The rate of female flowers is high and the fruit setting ability is strong.The fruit is of long rod shape with 26-35 cm in length,5-7 cm in diameter,and 1.1 cm in flesh thickness.Its pericarp is green and shiny.Its flesh tastes a little bitter. The single fruit weight is 400-500 g.It can yield about 45 t·hm
-2
.It is suitable to be cultivated in Fujian,Hubei and Sichuan Provinces.
2013 Vol. 1 (10): 103-105 [
Abstract
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622
) [
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1KB] [
PDF
911KB] (
696
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