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06 July 2013, Volume 1 Issue 14
    

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  • WANG Min, ZHANG Sheng-Ping, MIAO Han, LIU Shu-Lin, DONG Shao-Yun, WANG Ye, GU Xing-Fang
    . 2013, 1(14): 1.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Seed traits are closely related to plant growth and development,yield and quality
    of product organ,and environmental adaptability of plant.It is important to investigate thoroughly
    the inheritance and development regulation mechanism of seed traits for improving crop germplasm,
    increasing yield and solving the problem of biological energy s ource.In recent years,great progress has
    been made in studying Cucurbit seed traits.Studies on inheritance,development regulation mechanism
    and molecular biology of seed traits were reviewed in this paper. In addition,problems existing in
    current research were discussed and future research orientation was prospected.
  • SONG Bo, XU Hai, CHEN Long-Zheng, ZHANG Hui, KUANG Yuan-Yuan, YANG Jing-Hua, YUAN Xi-Han
    . 2013, 1(14): 9.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    This paper summarized research progress and achievements made in Brassica campestris
    L. ssp. chinensis(L.) Makino var. rosularis Tsen et Lee about its identification,evaluation of
    germplasm resources,new variety selection,breeding technology,cultivation technology,physiology
    and molecular biology technology.It also analyzed the problems existing in present studies and
    prospected the future research tasks for Brassica campestris L. ssp. chinensis(L.)Makino var. rosularis
    Tsen et Lee.
  • ZHOU Zhong-Li, XIA Ying, QU Song
    . 2013, 1(14): 17.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Taking 181 households of Xinjiang melon farmers as examples,on the basis of
    investigation data,this paper carried out descriptive statistical analysis on the factors influencing
    production and operating statusquo of melon. The paper adopted semilog multivariable linear regression
    method, and systematically analyzed the factors influencing melon production and operation benefits
    in Xinjiang,and the correlation between planting scale and benefit.The results indicate that the
    benefits are influenced by planting pattern,production mode,planting scale and region selection,etc.
    Accordingly,the paper puts forward suggestions in detail to improve the melon production and operation
    benefits in Xinjiang.
  • WANG Zhao-Ji, GAO Peng, LUAN Fei-Shi, LIU Shi
    . 2013, 1(14): 25.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Taking round shape watermelon line‘ Huayuanmuben’ as female parent and oval
    shape line‘LSW-177’as male parent,we crossed them and obtained F2 population constructed with
    180 single plants.We measured the length and width of the ripened watermelon〔Citrullus lanatus
    (Thunb.)Matsum. et Nakai〕,obtained fruit shape index from the ratio between fruit length and width,
    constructed the genetic map and analyzed the QTLs of relevant characteristics.The constructed genetic
    map contains 45 SSR-markers belonging to 13 linkage groups.The covering genome length was 547.3
    cM.The average distance between markers was 12.44 cM.The one QTL closely associated with fruit
    shape index was located in the 3rd linkage group between MCPI-12 and CMN05_69.This QTL was
    named as Fsi3.1.Its contribution rate was 2.8%,and its additive effect was negative,which showed
    synergia additive effect in reducing fruit shape index.
  • ZHAO Xin, WANG Yong, LAN Qing-Kuo, HE Chang-Zheng, CHEN Rui, LI 欧Jing, LIU Na
    . 2013, 1(14): 31.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In this research,10 Chinese cabbage〔Brassica campestris L. ssp. pekinensis(Lour)
    Olsson〕hybrids and their parents were taken as study objects.Based on the EST sequences from
    NCBI genome data base,a SNP site and multiplex EST- SSR sites,which could be used to identify the
    purity of Chinese cabbage hybrids,were screened by SSR marker and high resolution melting(HRM)
    technology.According to the requirement of seed purity identification by high throughput molecular
    technology,the key techniques such as seedling culture condition of single seed,extraction status of
    single grain plant, rapid DNA extraction method and establishment of multiplex PCR system were groped
    and optimized.Results showed that the single germinating seed after 48 h could gain DNA with higher
    quality by the improved CTAB method in 3 h. Meanwhile,plant leaves reached seeding stage after 7
    d,could complete DNA extraction by chexe-100 method within 40 min.The SNP site obtained by screening could be used to indentify the purity of 6 Chinese cabbage hybrids,and the multiplex ESTSSR
    locus could be used to indentify the purity of 10 Chinese c abbage hybrids.
  • ZHENG Xiao-Xu, YANG Feng-Shan, ZHU Xun, ZHANG You-Jun
    . 2013, 1(14): 39.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In this study,we constructed a recombinant of Plutella xylostella cadherin gene
    fragment.In order to enhance its expression in Escherichia coli,we studied the effect of time,
    temperature and IPTG concentration on fusion protein expression and its solubility.The results showed
    that the fusion protein gave rise to the maximum expression when host cells were cultured in LB at 37 ℃
    for 1.5 h and then induced by 0.1 mmol·L-1 IPTG at 25℃ 180 r·min-1 for 36 h.And the temperature
    change had little impact on the solubility of fusion proteins. SDS-PAGE test also indicated that the
    molecular weight of fusion proteins was the same as expected,wh ich is about 16 kDa.
  • ZHANG Nian, WANG Zhi-Min, YU Xiao-Hu, DING Ze-Qin, TANG Qing-Lin, TIAN Shi-Bing, WANG Yong-Qing
    . 2013, 1(14): 46.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    This study evaluated the level of genetic diversity of eggplant(Solanum melongena
    L.) germplasm resources through making morphological markers on 76 copies of eggplant germplasm
    resources. The results showed that the morphological traits of the tested eggplant germplasm showed
    genetic differences. The average variable coefficient between each germplasm traits was 54.83%,of
    which the largest variable coefficient was leaf spines,493.3%,the least variable coefficient was fruit
    curvature,10.9%. Clustering analysis was carried out on eggplant phenotypic character,76 eggplant
    materials were divided into 3 groups taking Pearson correlation coefficient 23 as clustering threshold,if
    taking Pearson correlation coefficient 19.5 as clustering threshold,76 eggplant materials were divided
    into 7 sub-groups.
    Key words:Eggplant;
  • ZHAO  He, WANG Xiao-Xuan, DU Yong-Chen, GAO Jian-Chang, GUO Yan-Mei
    . 2013, 1(14): 53.
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    Abstract:In this paper,cultivated tomato Lycopersicon esculentum Mill. 1052 was used as female
    parent and wild tomato L. peruvianum LA3858 was used as male parent.One F1 hybrid was obtained
    by embryo rescuing method.The hybrid was backcrossed with Lycopersicon esculentum Mill. 1052 to
    generate BC1 progeny.And 6 single plants of backcross F1 species hybrids were obtained. Moreover,129
    primers of CAPS,SSR and InDel on 12 chromosomes were used to analyze chromosome fragments of BC1.
    The results showed that these 6 BC 1 plants contained the whole genome of L. peruvianum LA3858.
  • SHAO Qin, YU Ze-Yuan, LI Xing-Guo, LI Wei, GAO Yan-Juan
    . 2013, 1(14): 59.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Xantha mutant 9388-1,a spontaneous mutant,was screened out from a normal green
    leaf melon(Cucumis melo L.) variety‘ Baishami No.1’.Its contents of photosynthetic pigment and Chl
    synthesis precursors,chlorophyll fluorescence kinetic parameters and ultra-structure of chloroplasts
    were studied with mutant parent‘ Baishami No.1’ as contrast.The results showed that photosynthetic
    pigment content in mutant leaves was significantly lower than that in‘ Baishami No.1’,while the ratio
    between chlorophyll a and chlorophyll b was significantly higher than in‘ Baishami No.1’.Chlorophyll
    biosynthesis was hampered at conversion from prophobilinogen(PBG) into uroporphyrinogen
    (Urogen Ⅲ).Compared with chlorophyll fluorescence kinetic parameters of‘Baishami No.1’,F0
    Pn,ФPS Ⅱ and ETR were significantly lower,while qN and NPQ were notably higher than that of
    ‘Baishami No.1’.There were no significant differences in Fm,FV/Fm and qP .Under transmission
    electron microscopy,9388-1 leaves were found with underdeveloped chloroplast,containing aberrant
    thylakiods,fewer graum and starch grains than that of‘Baishami No.1’.Grana lamellae were deranged
    with a line shape and less stacked,but grana lamellae was crowed together in normal one.These
    results indicated that the biosynthesis chlorophyll was hampered,leading to aberrant chloroplast and the
    photosynthetic pigments content changed,and then affected their stability.The mutant kept a higher
    heat dissipation capacity,which could prevent photosynthetic apparatus from damaged by excessive
    light energy and maintain the normal growth of plant.
  • MA Yan, LI Fang, SUN Zhou-Ping, SHEN Xiang-Jun, MA Jian
    . 2013, 1(14): 66.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Taking cucumber(Cucumis sativus L.) cultivar‘ Jinyou 30’as testing material,
    corn stalk∶cow dung∶slag=3V∶1V∶1V as cultivation medium,taken permeable woven bag,
    impermeable woven bag,nonwoven and double color film as 4 treatment bag materials,we investigated
    different bag materials effects on growth,xylem sap,fruit yield and quality of cucumber cultivated
    in bags in greenhouse. The results showed that after transplanting 40 days and 55 days, the height of
    cucumber cultivated in impermeable bags were significantly higher than that of other 3 treatments.The
    fresh weight of above-ground and dry weight of under-ground,and the nutrient content in cucumber
    xylem sap was also significantly higher than the other 3 treatments.Moreover,the fruit contents of
    soluble protein and VC were also higher than that of the other treatments.The results indicated that the
    impermeable woven bag was the optimum bag material for cucumber cultivated in bag.
  • SONG Zhi-Gang, YU Hong-Jun, JIANG Wei-Jie, ZHANG Ye, YANG Xue-Yong
    . 2013, 1(14): 72.
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    Taking‘Zhongza 106’tomato(Lycopersicon esculentum Mill.)as experiment
    material,and traditional peat and vermiculite media as contrast,this experiment studied on the
    physical and chemical properties and effect of tomato seedling culture with different mixing proportion
    substrates of decomposed straw,rice husk and sand.The results indicated that the mixing proportion
    substrates of straw could meet the requirement of seedling media and make the quality of tomato seedling
    better than that of the traditional peat and vermiculite substrates.Among all treatments,T1(100%
    straw) and T2(straw:rice husk=75V∶25V) compound substrates could culture tomato seedling with
    the best quality.Its physiological index was notably superior t o the other treatments and contrast.
  • ZHANG Jian, WU Zhi-Gang, ZHAO  Xin, MA Ming, NING Wei
    . 2013, 1(14): 78.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Counting root tip chromosome numbers,artificial pollination experiments and flow
    cytometry,etc. were used to confirm that Taraxacum coreanum Nakai was tetraploid. Artificial
    pollination experiments showed that Taraxacum coreanum reproductive type was facultative apomixes,
    and apomixes rate was around 50%.The result of testing by flow cytometer showed that the ratio of seed
    cell ploidy level was 2C∶3C∶4C.The reproductive type of tetraploid T. coreanum was facultative
    apomixes with independent endosperm development.
  • ZHANG Fu-Jin, ZHANG Xin-Xin, LI Guo-Yin, FENG Xiao-Hui, REN Chao, LUO Hong, GAO Tian-Yun
    . 2013, 1(14): 81.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Mathematical statistic analysis was carried out on 6 800 samples of veggies,fruits,
    beans,nuts and seeds produced in Inner Mongolia. The results showed that the dominated vegetables
    in Inner Mongolia were green leaf vegetables,potato family vegetables and melons,and their yields accounted for 70% of the total vegetables production. Since 2003,with the rapid increase of
    detected vegetable numbers,the status of vegetable quality safety had entered into a new stage of
    sustainable steady after a rapid decline or periodic fluctuation,and the differences in safety levels
    were obvious,of which the range of exceeding standard rate of pesticide residues in eggplants,melons
    and beans(about 55% of the total vegetables)was under 2%,with a mean of 1.1%,1.6% and
    3.1%,respectively. They belonged to stable type. The exceeding standard rate of pesticide residues
    in green leaf vegetables and Chinese cabbage(about 30% of total vegetables) presented a decreasing
    trend year by year,wondering in the range of 7%-10%.They belonged to risk main body type.The
    time period of exceeding standard of pesticide residues in root vegetables and cabbage was under
    predictable,and in some years its exceeding standard rate was over 5%(amplitude variation was
    over 15 percentage points).They belonged to random risk type.The kinds of pesticides,which
    exceeding standard rate in vegetables were relatively intensive,presented variance in different
    vegetable species.70% of the pesticides belonged to intermittent over-limits and the probability was
    below 5%.About 20% tradition pesticides often exceed standard and their probabilities were over
    30%.The exceeding standard of organophosphorus and carbamates pesticides in vegetables presented a
    decreasing tend,while the risk of some new type pesticides containing nitrogen atoms and high effective
    pyrethroid insecticide showed an increasing tendency.
  • SUN Xing-Xiang, LIN Hong-Mei, HE Chun-Ping, NI Wei
    . 2013, 1(14): 88.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Fusarium wilt is an important factor causing clogging in continuous cultivation of
    watermelon〔Citrullus lanatus(Thunb.)Matsum. et Nakai〕.In production,we generally adopt crop
    rotation,grafting,biological control and medical treatment to prevent this disease.This experiment
    studied on many controlling methods,such as grafting on different stock varieties,using biological
    pesticides and biological fertilizer,etc.at different plot of land during the period of watermelon
    continuous cultivation.The results indicated that the effect of biological control was not obvious,if
    the continuous cultivation period was long,and watermelon was planted in the fields where Fusarium
    wilt occurred seriously. Biological control could only play an inhabiting role in dome extent,while the
    grafted seedling had remarkable effect,the control effect is 100.0%.If the continuous cultivation
    period was short,and the plot had slightly suffered from Fusarium wilt,it was efficient to apply BIO
    biological fertilizer or grafting.
  • HE Run-Yun, ZHOU Ai-Zhu, CHEN Xiao-Zhen, LI Hai-Ding
    . 2013, 1(14): 92.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Taking 20% Bismerthiazol WP,72% Streptomycin WP,77% Kocide WP a s experiment
    materials,we found that 20% Bismerthiazol WP mixed with 72% Streptomycin WP could prevent and
    cure pepper bacterial wilt.The average control effect could reach 96.51% with better stability,which is
    worthwhile to extend.
  • CHEN Qing-Qi, MEN Wan-Jie, LEI  Na
    . 2013, 1(14): 95.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    ‘Hajiao’ is a new cherry tomato F1 hybrid developed by crossing inbred lines 5-121212
    as female parent and 8-322311 as male parent.This variety belongs to semi-unlimited growth type
    .The plant is dark green in color with single raceme and of common leaf shape.It has yellow flower,
    pink and long round fruit with sharp.The single fruit weight is 15-20 g.Its growing period is about 110
    days.Its fruit tastes sweet and sour.It is mid-resistant to leaf mold and virus disease,and is suitable
    for protected cultivation. It can produce about 52.5 t·hm -2.
  • LI Gao-Feng, WEN Guo-Hong, LI Jian-Wu, WANG Yi-Hang, ZHANG Rong
    . 2013, 1(14): 97.
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    Longshu No.11’ is a new potato variety selected from L9712-2(92-29-81×93-1-204)
    as female parent with‘ Yuanza 22’ as male parent.It is of late maturity. Its growing period is about
    124 days. Its tuber is round with yellow micro-textured peel,yellow flesh and medium shallow eyes.
    The average content of dry matter,starch,crude protein,VC and reducing sugar is 23.7%,18.68%,
    3.16%,144.2 mg·kg-1 and 0.162%,respectively.It has higher resistance to late bligh t than‘ Longshu
    No.5’and has better resistance to leaf roll virus in field.It is sui table for open field cultivation in highaltitude,
    cold and wet,and semi-arid region. It can yield about 20.25 t·hm-2.
  • ZHOU Quan, ZHU Bie-Fang, MA Lu-Ping, YI Xue-Sai, ZHOU Jian, PENG Qi-Feng
    . 2013, 1(14): 100.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Shenyu’ is a new watermelon variety with small and medium size fruit,yellow flesh,
    and seedless.The whole growth period of this variety is about 100 days.It takes about 30 days from
    female flower bloom to fruit mature.The plant grows in medium and strong vigor.Cauline leaf is in
    medium level.It has strong resistance to disease and stress. The fruit is of short oval shape with dark
    green peel.The thickness of fruit peel is about 1.0 cm.The fruit has bright yellow flesh,tastes crispy
    and refreshing with less fiber. Its seedless performance is good.The central soluble solid content is
    11.5%-13.5%.Edge soluble solid content is 7.5%.Its single fruit weight is 2-4 kg.It can normally
    yield about 27-30 t·hm -2.
  • ZHANG Li-Rong, HUANG You-Cheng, HAO  Kai, ZHOU Ji-Bing, CHEN Xiao-Jun, WU Guo-Jing
    . 2013, 1(14): 103.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    ‘Gantiannuo No.3’ was a new colored waxy corn bred by crossing double heterozygote
    (J38×J40)as female parent and sweet waxy inbred line‘Tiannuo 7’as male parent. J38 and J40 were
    waxy corn with white seed coat and purple seed coat,respectively.The sweet waxy inbred line‘ Tiannuo
    7’carries the homozyogous super sweet and waxy genes.‘Gantiannuo No.3’ is a new table corn.Its
    plant height is 232 cm,with 105 cm ear height. It is resistant to lodging with good stay-green ability and
    wide adaptation. Its edable quality is superior.The ear shape is mainly of cone type. The fresh ear can
    yield about 16.5 t·hm-2.It takes about 106 days from planting to harvesting.‘Gantiannuo No.3’is
    suitable to be planted at spring maize area in Northwest China.