01 May 2014, Volume 1 Issue 5

    

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  Research Progress on Botrytis cinerea Resistance to Different Fungicides

  HAN Zhi-Qi, BEN Hai-Yan, XIE Xue-Wen, SHI Yan-Xia, LI Bao-Ju

  . 2014, 1(5): 6.

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  Botrytis cinerea is a phytopathogenic fungi responsible for grey mould，which is an important
  disease in many species of fruit trees，vegetables and ornamental crops. Recently，B. cinerea has developed
  increasing severe resistance to a broad range of fungicides. This paper summarizes the research progress about B.
  cinerea at various host plant’s resistance to drugs，their resistance mechanism and methods for detecting tolerant
  bacteria reported at home and abroad. It also predicts the future orientation of this study.
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  Construction of Cherry Tomato Fruit Specific LYC-B Interference Vector and
  Validation of Its Specific Expression in Fruits of Discrepant Colors

  WANG Qiao-Li, LIANG Yan, ZHANG Zhen-Cai, LI Cui, LI Yun-Zhou, WANG Ling-Hui

  . 2014, 1(5): 11.

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  The experiment will be conducted to research the specific impacts of lycopene β-cyclase
  interference on carotenoid metabolism，cherry tomato（Solanum lycopersicum L. var. cerasiforme Alef.） fruitspecific
  lycopene β-cyclase interference vector was constructed，and the validity in different color fruits were
  verified. Cherry tomato fruit-specific promoter E8 was amplified according to X13437.1 and fruit-specific vector
  of E8-pBI121 was constructed，which could be expressed in cherry tomato fruits of pink，red，green and purple
  colors. According to X86452.1，two different lycopene β-cyclase sequences B1 and B2 were amplified，LYC-B1
  with 801 bp from 61 bp to 861 bp and LYC-B2 with 302 bp from 480 bp to 781 bp of LYC-B. The interference vector
  of pBI121-B1B2 with CaMV 35S promotor and the fruit-specific expression interference vectors of E8- pBI121-
  B1B2 with E8 promotor were constructed. E8-pBI121，pBI121-B1B2 and E8-pBI121-B1B2 were transferred
  into Agrobacterium tumefaciens GV3101 with the freeze-thaw method，then transformed into living cherry tomato
  fruits and leaves by injection. The results of GUS staining 7 days after injection showed that E8-pBI121 expressed
  in pink，red，green and purple cherry tomato fruits；pBI121-B1B2 expressed in leaves，fruits and seeds，while
  E8- pBI121-B1B2 expressed just in fruits and seeds.
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  Toxin-producing Condition Optimization and Biological Activity Determination
  of Colletotrichum orbiculare

  TANG Shuang-Shuang, LIU Zhi-Heng, YU Chao-Ge, ZHENG Chuan, LI Jian-Bing, ZHAO Ting-Chang

  . 2014, 1(5): 19.

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  Toxin-producing condition optimization and determination of crude toxin biological activity
  and its physical，chemical properties were carried out on Colletotrichum orbiculare. The results showed that
  different culture conditions had different influences on pathogenic toxin activity. The Richard culture medium with
  pH 6 ，culture temperature at 25℃，in dark，and shaking continuously for 20 d were the best toxin-producing
  conditions. The watermelon stem inoculated by crude toxin could produce the same acute symptom as the bacteria
  did，which had inhibitory effect on seed radicle growth and seed germination； and also a collapsing role on
  seedling. That toxin is the main pathogenic factor in the process of watermelon anthrax bacteria infection. Among
  the 10 kinds of selected plants inoculated by crude toxin，the symptoms of muskmelon，summer squash and
  strawberry were significant，while no symptoms were seen on tomato，eggplant and pepper.
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  SRAP Analysis of Mustard Germplasm Genetic Diversity and Genetic
  Relationship

  LI Ning, SI Jun, REN Xue-Song, SONG Hong-Yuan, LI Cheng-Qiong

  . 2014, 1(5): 26.

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  Abstract：The paper analyzed the genetic diversity of 111 mustards〔 Brassica juncea（L.）Czern. et Coss.〕
  materials using SRAP markers，and recorded their morphological characteristics．21 primer combinations with high
  polymorphism were screened from 300 SRAP primer combinations．106 polymorphic bands among 150 bands were
  amplified by 21 SRAP primer combinations，and the polymorphic rate was 70.67%．The SRAP clustering results
  showed that the genetic similarity of 111 mustards ranged from 0.38 to 0.89，and 111 mustards were divided into
  5 groups and several subgroups．The agronomic traits including leaf blade traits，and root，stem，leaf，bolting
  development were investigated and analyzed．The results showed that the genetic similarity of 111 mustards ranged
  from 0.39 to 1.00，and they could be divided into 4 groups and several subgroups．These two results were nearly the
  same，indicating that SRAP markers could be applied in studying the genetic diversity and relationships of mustard
  germplasm resources．
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  Effects of Saline Stress on Growth and Rhizoplane Soil Enzyme Activities of
  Cucumber Seedling Grafted on Different Rootstocks

  ZHAO Yuan, WU Feng-Zhi

  . 2014, 1(5): 33.

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  Based on the saline content and composition of the soda saline soil in Heilongjiang and other
  Provinces，this experiment simulated its saline composition，adopted pot culture，grafted cucumber（ Cucumis
  sativus） seedlings onto 2 salt-tolerant rootstocks，and 2 salt-sensitive rootstocks as experimental materials. Ownroot
  seedlings were taken as the contrast. Saline solution（100 mmol·L^-1，pH 9.0） was used to treat the soil. We
  studied the effects of salt stress on growth and soil enzyme activities of cucumber seedlings grafted on these different
  rootstocks. The results indicated that the yield per plant，the ground fresh weight，the root fresh weight，the plant
  height and the chlorophyll content of cucumber seedlings grafted on 2 salt-tolerant rootstocks were significantly
  higher than that of the 2 salt-sensitive rootstocks and own-root cucumber. The soil urease，neutral phosphatase
  activities after transplanted 30 d and the catalase，dehydrogenase activities after transplanted 40 d of cucumber
  seedlings grafted on salt-tolerant rootstocks were higher than that of salt-sensitive rootstocks and own-root plants.
  These 4 kinds of soil enzyme activities of cucumber seedlings grafted on salt-tolerant rootstocks after transplanted
  50 d were all higher than that of salt-sensitive rootstocks and own-root cucumber. Under soil salinization
  condition，cucumber seedlings grafted on salt-tolerant rootstocks grew robustly and suffered low degree of salt
  injury. That account for that graft can significantly improve cucumber salt resistance； the resistance enhancement of
  cucumber seedlings grafted on salt-tolerant rootstocks is closely related to the increase of soil enzyme activity.
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  Impact of Temperature on Virulence of Beauveria bassiana Canada No.1 Isolate to
  Bemisia tabaci（Gennadius）

  LIU Zhao, ZHANG Xian-Liang, LEI Zhong-Ren

  . 2014, 1(5): 39.

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  To identify the effects of temperature on the infection of Beauveria bassiana Cananda No.1
  isolate against the tobacco whitefly， Bemisia tabaci（Gennadius），the spore germination rate，mycelial growth
  speed，changes in activities of extracellular proteinase and chitinase were observed and studied under 5 different
  temperature conditions（19，22，25，28，31℃）. The results showed that the isolate Cananda No.1 owned
  the maximum parameter values and the highest virulence against B. tabaci at temperature of 28 ℃ . The spore
  germination rate，mycelial growth speed，activities of extracellular proteinase and chitinase，and virulence against B.
  Tabaci were significantly reduced when the temperature was higher or lower than 28 ℃ .
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  Effects of Copper on Black Sesame Spot and Total Phenol Content in Chinese
  Cabbage

  CHEN Qi-Wu, YANG Xiao-Yun, LUO Qing-Xi, ZANG Shun, ZHANG Shu-Xia, SI Chao-Guang, WANG Yuan, ZHANG Qing-Xia

  . 2014, 1(5): 43.

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  Taking Chinese cabbage〔 Brassica campestris L. ssp. pekinensis（Lour.）Olsson〕line 03B9
  with high sensitivity and C24 with high resistance to black sesame spot as experimental materials，this paper
  studies the effect of different copper concentration treatments on black sesame spot， and total phenol content
  and PPO activity. The results showed that the sesame spots of the 2 lines were markedly increased as the copper
  concentration increased，and the occurrance degree of 03B9 was significantly higher than that of C24. During the
  copper treatments，the total phenol content，PPO activity in the petiole of line C24 were higher than that in line
  03B9 with 25.6% and 77.0%，respectively. The preliminary tests showed that under hydroponic conditions，the
  occurrence of black sesame spot was promoted by the concentration of exogenous copper, the degree of disorder was
  related to the copper treatment concentration. Meanwhile, the total phenol content and PPO activity were influenced
  by copper.
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  A New Subgynoecious Autumn Cucumber F₁ Hybrid—‘Tangqiu 208’

  HAN Jing-Ling, LI Yu-Hua, SONG Rui-Sheng, HOU Bo-Sheng

  . 2014, 1(5): 47.

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  ‘Tangqiu 208’ is a new early maturing and subgynoecious autumn cucumber F₁ hybrid bred by
  crossing C18 as female parent and P107 as male parent. Its female flower rate is 85.17%. The commercial fruit is
  14.63 cm in length and 3.97 cm in diameter. The average single fruit weight is 147 g. Its fruit skin is green and
  shinning with larger and dilute tubers. The fruit has good commercial character. Its VC content is 87.0 mg·kg^-1. Its
  yield is about 75 t · hm^-2. It is suitable for protected and open field cultivation in spring and autumn. It is suitable
  to be planted in Hebei，Liaoning Provinces and Tianjin，etc.
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  A New Hot Pepper F1 Hybrid—‘Yumeiren’

  ZHAO Xiang-Mei, GUO Jing, ZHANG Jian-Guo, CHEN Man, SHEN Ai-Min

  . 2014, 1(5): 49.

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  ‘Yumeiren’ is a mid-early maturing hot pepper F₁ hybrid bred by crossing X-01-7-2-2 as female
  parent and X-08-3-4-1-1 as male parent. The plant grows vigorously. The first flower sets on the 10.6 node. The
  young maturing fruit is yellow green and the old matured fruit is bright red with good commercial quality and hot
  flavor. Its fruit is long lanceolate，with 22.0 cm in length and 1.5 cm in diameter. The single fruit weight is 15-
  20 g. Its capsaicin content is 53.60 mg·kg^-1 and the VC content is 895 mg·kg^-1. It has higher resistance to virus
  disease and phytophthora blight than that of the contrast‘ Xiangla No. 4’. It can yield 45-60 t·hm^-2. It is suitable
  to be grown in protected and open fields in spring and autumn in Henan Province and surrounding areas.
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  A New Sweet Pepper F₁ Hybrid—‘Zhangtianjiao No.1’

  WANG Ai-Wen, ZHONG Hong-Qing, YAN Ming, XUE Long, HAN Shun-Bin, HUA Jun, SUN Bing-Ling

  . 2014, 1(5): 52.

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  ‘Zhangtianjiao No. 1’ is a new sweet pepper F₁ hybrid developed by crossing inbred line Y03-
  167 as female parent and Y03-96 as male parent. It takes 75-85 days from planting to harvesting. The first bud is
  borne at node 12-14. It is of mid-late maturity. The matured fruit is of square lantern shape，with 3 - 4 lobes，
  0.7-0.9 cm in pulp thickness，10-11 cm in length and 9-11 cm in diameter. The matured fruit is green in color
  with smooth surface. The single fruit weight is 200-260 g. It is tolerant to storage and transportation. It has high fruit
  setting rate. Its fruit shape symmetry is beautiful. It has good commodity. Its normal yield can reach 81.0 t· hm^-2. It
  is suitable for cultivation in open fields at Hexi of Gansu Province and Linhe of Inner Mongolia Autonomous Region.
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  A New Muskmelon F₁ Hybrid—‘Dunmi No. 1’

  MA Wen-Hai, XI Xiao-Fei, XU Hui-Xin

  . 2014, 1(5): 54.

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  ‘Dunmi No.1’ is a new muskmelon F₁ hybrid developed by crossing 2 inbreed lines ML29 as
  female and ML20 as male parent．It is of mid-early maturity. Its growth period takes about 87 days in open field
  cultivation. The fruit development period is about 40 days. The plant grows vigorously. Its leaf is dark green in color.
  The fruit shape is oval and index is 1.4. The fruit skin is pure white with overlapping curve. Its pulp is light green
  in color and the pulp thickness is 3.4 cm. The central soluble solid content is 16.4%. It is suitable for storage and
  transportation. The single fruit weight is about 1.5 kg and its average yield is about 33.9 t·hm^-2. It is resistant to
  melon Fusarium wilt，downy mildew and powdery mildew. It is suitable to be cultivated in Jiuquan and similar
  areas of Gansu Province.