01 May 2015, Volume 1 Issue 5

    

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  Research Progress on Early Maturity Breeding of Fruit Vegetables

  QIAO 　Jun, SHI 　Yao, WANG Li-Ying

  . 2015, 1(5): 14.

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  Early maturity is an important index for fruit vegetables breeding，and it is of great significance for increasing agricultural production and farmers’ income. This paper reviewed the evaluation indexes of early maturity breeding，the agronomic characteristics related to early maturity，and breeding methods for early maturity. The paper also prospected the possibility of using genetic engineering to assist early maturity breeding.
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  QTL Mapping for Sourness-associated Traits in Melon（Cucumis melo L.）

  ZHU Hui-Qin, FU Qiu-Shi, YI Hong-Ping, WANG Huai-Song

  . 2015, 1(5): 18.

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  A F2 population including 153 individuals derived from a cross between 2 cultivars‘ 60’ and ‘61’，which were acidless and acid respectively，was used to analyze a linkage of SSR markers and construct a genetic map. The map contains 15 linkage groups spanning 923.2 cM with an average of 8.71 cM between markers. The multiple QTL model（MQM）method of software package MapQTL version 4.0 was used to map and analyze QTLs. 3 QTLs were detected for citric acid content，including one major QTL（expl≥10%） named cit 8.1，which explained 34.8% of the phenotypic variation. 4 QTLs were detected for TA，among which one major QTL named ta 8.1，which explained 47.5% of the phenotypic variation. 2 QTLs were detected for pH，among which one major QTL named ph 8.1，which explained 82.7% of the phenotypic variation. These 3 major QTLs related with acdic properties were co-located on LG Ⅷ .
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  Effect of Different Storage Temperature on Transplant Quality of Broccoli Plug Seeding

  PAN Xiao-Bing, TIAN Yong-Qiang, LI Juan-Qi, YANG 　Tian, GAO Li-Hong

  . 2015, 1(5): 23.

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  To study the effect of different storage temperatures on broccoli（Brassica oleracea L.var. italica Plenck）seedling quality，growth and development after fix planting，this paper screened the related morphological indexes of seedling new heart leaf growth rate after planting，taking‘good’as test material to evaluate the quality of broccoli seedlings under temperature 11，16，21 ℃ and stored for 0，2，4，6 days，respectively．The results
  showed that in the storage temperature of 11 ℃，seedling’s morphanological index maintained in good shape，the chlorophyll content per unit area was relatively higher，and the seedling new heart leaf grew rapidly．The correlation analysis results showed that the sound seedling index and plant recovery ability after fix planting during storage period had the highest correlation coefficient．The sound seedling index could be an indicator for evaluating
  the storage quality of the broccoli seedling．The path analysis results showed that composite indexes of chlorophyll content per unit area，sound seedling index，specific leaf weight could well reflect the growth recovery ability of broccoli seedling after short-term storage．
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  Cloning and Expression of SRK2F Gene in Chinese Cabbage

  ZHOU Zhi-Guo, WANG Cong-Yan

  . 2015, 1(5): 28.

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  Taking non salt and drought tolerant inbred lines of Chinese Cabbage〔Brassica campestris L.spp. pekinensis（Lour）Olsson〕SY-14-06 as material，extracted total RNA from root，and reverse transcribed cDNA．According to SRK2F gene sequence of Brassica rapa，primers were designed and 1 044 bp open reading frame（ORF）was cloned．The SRK2F protein contained 347 aminoacids，with a prediction molecular weight of 39.3 kD and PI of 4.88．We construct the procayotic expressive plasmids pEASY-E1-SRK2F using pEASY-E1 vector．After transformation to Transetta（DE3），the expression of recombinant proteins were detected with SDSPAGE．The structural analysis of SRK2F though Smart-embl showed that it contained silk threonine structural domain，which was located at the position of 4-260 aminoacid residues．The ClustalX2 comparison indicated that the SRK2F had close genetic relationship with Arabidopsis thaliana．Finally，purified this protein by affinity chromatography medium，which was known as Nickel ion metal affinity chromatography medium．Thus，gained the purified fusion protein.
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  Effects of Inoculations of Glomus mosseae and/or Rhizobium on Potted Bean Growth

  LI Ya-Hui, GAI Jing-苹, CHEN 　Qing, LI Zhi-Fang

  . 2015, 1(5): 33.

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  Under greenhouse condition，common bean（Phaseolus vulgaris Linn.）‘Taiguowujindidou’was sowed in pots to estimate the effects of different inoculation ways on bean’s plant growth，nodulation，total pod weight of single plant，AM colonization rate，nitrogen and phosphorus contents and absorption of bean plant. When seeding，the beans were inoculated with Glomus mosseae（AM），rhizobia（RH），Glomus mosseae and
  rhizobia（AM+RH），and the beans without inoculation were taken as the control（CK）. The results indicated that dual inoculation（AM+RH） could obviously promote the plant growth of beans. Compared with inoculation with Glomus mosseae（AM），rhizobia（RH）and none（CK），the plant total dry weight of the beans inoculated with AM+RH increased 34.3%， 82.3% and 57.9%， respectively. Besides，the dual inoculation could increase the total pod weight of single plant and promote bean’s absorption of nitrogen and phosphorus. The phosphorus absorption of total plant was increased by 42.1%， 314.8% and 325.4%， respectively than that by AM，RH and CK. While，the nitrogen absorption of total plant was increased by 36.8%，45.1% and 43.4%，respectively than that by AM，RH and CK. The Glomus mosseae and rhizobia had synergistic effect on common bean，and the Glomus mosseae could effectively improve bean’s resistance，reduce the yellow leaf ratio，and thus decrease the occurrence of diseases.
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  Effect of Soil Microbial Communities in Continuous Cropping and Rotated Cropping Systems on Cucumber Seedling Growth and Soil Enzyme Activity

  LIU 　Jie, WU Feng-Zhi, ZHOU Xin-Gang

  . 2015, 1(5): 38.

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  This paper studied the effects of microorganism population in continuous and rotated cropping on cucumber seedling growth and soil enzyme activity. The results showed that in rotated cropping microbial treatment，the fresh weight，chlorophyll content，the activities of neutral phosphatase and invertase of cucumber seedlings were significantly higher than that in continuous cropping microbial treatment. Both the continuous and rotated cropping microbial treatments had increased the fresh and dry weights，chlorophyll contents，total leaf area of cucumber seedling，and significantly increased the activities of soil neutral phosphatase，invertase，urease. Summing up the above，soil microorganism population in both cropping systems had a positive effect on the growth of cucumber seedling，and the positive effect on soil microorganism population in rotated cropping was stronger than that in continuous cropping.
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  Resistance Evaluation of Different Pepper Varieties to Meloidogyne incognita

  LIU Da-Wei, SUN Meng-Meng, LIU Xiu-Jie, WU Hai-Yan

  . 2015, 1(5): 42.

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  Resistance of 24 pepper（Capsicum annuum L.） cultivars to Meloidogyne incognita was identified by indoor pot inoculation method in this paper. The results showed that 11 pepper cultivars were highly resistant to Meloidogyne incognita，3 cultivars were moderately resistant，7 cultivars were resistant，and 3 cultivars were highly susceptive.
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  Effects of Different Nitrogen Concentration on Growth，Development and Quality of Hydroponic Chinese Chives

  DU Hong-Yan, YU Ping-Bin, JI Yan-Hai, WU Zhan-Hui, LIU Ming-Chi

  . 2015, 1(5): 45.

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  In order to determine the nitrogen concentration of nutrient solution suitable for hydroponic Chinese chives（Allium tuberosum Rottlerspreng）7 levels of Nitrogen concentration（4，6，8，10，12，14，16 mmol·L^-1）were set up to study the relevant product by using a new type of hydroponic system developed by Vegetable Research Center of Beijing Academy of Agriculture and Forestry Sciences. The growth index，individual dry and fresh weight
  and yields，physiological characteristics and nutritional quality were studied. The results showed that 8-12 mmol·L^-1 nitrogen concentration of nutrient solution are suitable for hydroponic Chinese chives. Among them，10 mmol·L^-1 Nitrogen concentration treatment showed the best effect on hydroponic Chinese chives growth index and yields； and 12 mmol·L^-1 nitrogen concentration treatment showed the best quality of hydroponic Chinese chives.
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  Effects of Cutting Stems Regeneration on Different Kinds Okra 〔Abelmoschus esculentus（Linnaeus）Moench〕

  LIU 　Yong, WAN San-Lian, WU Qian-Xing, LUO 　Feng, XIAO Ri-Sheng, KONG Xiang-Yi

  . 2015, 1(5): 49.

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  This study researched the cutting stems regeneration on the fruit characters，quality and the economic benefit of different kinds Okra〔Abelmoschus esculentus（Linnaeus）Moench〕（‘Taiwanwufu’，‘Lvyangjiao’，‘Dongjingwujiao’，‘Zaoshengwujiao’，‘Okra No.1’，‘Okra No.2’） . The results showed that the cutting stems regeneration technology was 40 days ahead of harvesting and could significant prolong the harvesting period nearly 30 days. In the synthetic features of 6 kinds Okra，the‘ Taiwanwufu’ was the best not only in the yield，fruit quality，but also in the economic benefit，which the income could reached at 8 800-9 750 RMB per 667 m²，and the everyday income at 62.0-68.7 RMB per 667 m².
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  Price Decomposition and Contribution to Price Fluctuation of Different Vegetables in China

  SHEN 　Chen, ZHANG Yu-Mei, LI Zhi-Qiang

  . 2015, 1(5): 52.

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  Using seasonal decomposition and HP filter method，this paper decomposed the wholesale prices of 28 vegetables into trend fluctuation，cyclical fluctuation，seasonal fluctuation and random fluctuation for more details of the characters and laws for different vegetables，and summarized the differences between different vegetables price fluctuation and changes. The results indicated that in recent years the impact of seasonal
  fluctuation to vegetables price fluctuation became gradually weaken，while the impact of random fluctuation to fruit and leafy vegetables was strengthened constantly. There were big differences in the influences brought by different fluctuation types on vegetable prices. Fruit vegetable price was more significantly affected by seasonal fluctuation.While leafy vegetable price was more easily affected by random fluctuation. Cyclical fluctuation had remarkable impacted to ginger and garlic price fluctuation.
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  A New Eggplant F1 Hybrid—‘Jingqie No. 13’

  QIAN Zong-Wei, CHEN Hai-Li, CUI Yan-Ling

  . 2015, 1(5): 58.

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  ‘Jingqie No. 13’ is a new eggplant F1 hybrid developed by crossing inbred line 1156 as female parent and 1125 as male parent. It is of early maturity. The first flower sets on the 10 th node. The plant has strong growth vigor with short internodes. Its fruit is of straight and long-cylinder shape. The fruit is black and shiny in color，25-30 cm in length，5-6 cm in diameter. It has strong continuous fruit setting ability. It can yield over 63.6t·hm^-2. It has strong tolerance to low temperature. It is suitable for protected cultivation in northern China.
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  A New Cucumber F1 Hybrid—‘Jinyou 401’

  HAN Yi-Ke, DU Sheng-Li, WEI Ai-Min, LIU 　Nan, CHEN Zheng-Wu, LI 　Ping

  . 2015, 1(5): 61.

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  New F1 hybrid cucumber‘ Jinyou 401’ was released by crossing an elite DH line J05-4 as female parent with inbred line 21-4-1 as male parent. The hybrid plant has strong growth vigor and medium sized green leaves. Its fruit is mainly set on the main stem. It has strong continuous fruit-setting ability. The fruit is dark green in color and very glossy，about 35 cm in length and 200 g in weight. It has high rate of commercial fruits.It is resistant to downy mildew，powdery mildew，Fusarium wilt and virus，etc. Its yield in spring open field cultivation is about 82.5 t·hm-2. It is suitable for open field and plastic tunnel cultivation in fall in Hebei，Henan，Sichuan，and northeast China.
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  A New Pepper F1 Hybrid —‘ Haifeng 1052’

  ZHANG Shu-Gen, XING Yong-Ping, ZHANG Jun-Min, WANG Zhen-Quan, DENG Xiao-Mei, JIANG Zhong-Ren, LI Chun-Ling

  . 2015, 1(5): 63.

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  ‘Haifeng 1052’ is a new pepper F₁ hybrid with early maturity developed by crossing double haploid line 08-123 as female parent with inbred line F07-253-2-1 as male parent. The first flower sets at 7-8 ^th node. Its fruit has smooth surface and slightly wrinkled shoulder. The fruit is 31.2 cm in length，1.5 cm in width，and 32.6 g in weight．The fruit has strong spicy flavor and light green peel. The matured fruit has bright red peel. The contents
  of VC，soluble sugar，protein and capsaicin are 672 mg·kg^-1，1.7 g·kg^-1，14.0 g·kg^-1 and 0.25 g·kg^-1，respectively. It is resistant to TMV and CMV，and mid-resistant to Phytophthora blight. Its average yield is about 45 t·hm^-2．It is suitable to be cultivated in protected and open fields in northern China，and in open fields in Guangdong，Guangxi，Shanxi and Shaanxi Provinces，etc．
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  A New Muskmelon F1 Hybrid—‘Xintian No. 1’

  LIU Xi-Cun, WANG Wen-Ying, DONG Yan-Qi

  . 2015, 1(5): 65.

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  ‘Xintian No.1’ is a new muskmelon F1 hybrid developed by crossing 2 inbreed lines TM-98-07 as female parent and TF-97-112 as male parent．It is of mid-early maturity. Its growth period takes about 85 to 120 days. The fruit development period is about 33 days. It has strong growth vigor. It has resistance to ZYMV and powdery mildew，mid-resistance to Fusarium wilt．Its fruits are of round shape with white skin. Its flesh is orange in color and the thickness is 3.5-4.0 cm. Its soluble sugar content is 16.0%-18.5% and has a little gradient between its center and fringe. The average single fruit weight is 1.8 kg. It can yield 52.5-60.0 t·hm-2. It is suitable for open field and protected cultivation in Henan Province.