ISSN 1000-6346
CN 11-2326/S
中华人民共和国农业农村部 主管
中国农业科学院蔬菜花卉研究所 主办
CN 11-2326/S
中华人民共和国农业农村部 主管
中国农业科学院蔬菜花卉研究所 主办
Sowbane mosaic virus（SoMV）is one of the most important diseases for spinach．SoMV can be
transmitted by seed，pollen，insect vector and mechanical manners．Along with the further expantion in imported
quantities of seeds and seedlings，and planting industrialization，the risk of SoMV introducing into China is
increasing．This paper reviewed the biological characteristics，distribution，host range，endanger sympton，
transmission route，and detecting techniques of SoMV．The paper also summarized the potential danger and risk
of SoMV coming into China．
The Bt gene，Cry2Aa2，were transferred into Chinese kale（Brassica alboglabra L. H.
Bailey）by biolistic and agrobacterium-mediated transformation methods and these 2 transformation systems were
optimized and compared．High transformation rate was obtained by bombarding Chinese kale callus with a distance
of 6 cm and for one time．The transformation efficiency was 0.43%. The suitable procedure for agrobacteriummediated
transformation method in Chinese kale was using hypocotyl as material，pre-culture for 2 days，infected
by agrobacterium for 10 minutes，co-culture for 2 days，antibacterial-culture for 5 days and then screened in
selective culture with 5 mg·L-1 kanamycin．The transformation efficiency was 0.37%．Identified by PCR，PCRSouthern
blot analyses，the target gene were transferred into the Chinese kale by both biolistic and agrobacteriummediated
transformation methods．Insect resistance identification proved that the positive transgenic Chinese kale
plants had stronger resistance to Mamestra brassicae larva than the non-transgenic plants.
Photosynthesis is one of the plant relatively sensitive physiological metabolism reaction to
salt stress．This experiment took 2 cucumber varieties‘ Zhongnongda No.22’ and‘ Deltastar’ with different
sensitiveness to salt stress as material to study photosynthetic responses to salt stress． The results showed that the
photosynthetic pigment（chlorophyll and carotenoid）contents，net photosynthetic rate，dark respiration rate and
transpiration rate of cucumber seedlings decreased significantly under salt stress．The number of thylakoid lamella
of chloroplast was reduced greatly and starch grains became fewer and smaller when cucumber seedling treated
with salt stress．With the process of salt stress，the expression levels of rbcL，encoding the large subunits of
Rubisco（ ribulose-1，5-bishosphate arboxylase/oxygenase）and Csppc2，encoding PEPC（phosphoenolpyruvate
carboxylase） increased first then decreased，while the enzyme activity of Rubisco and PEPC declined
remarkably．Compared with‘ Zhongnongda No.22’，‘Deltastar’ had better tolerance to salt stress．
Taking 4 different cucumber varieties as material，this study tested chlorophyll content changes
in cucumber leaves under different low light treatments，explored the most appropriate light intensity and best
appraisal period for low light tolerance identification during cucumber seedling stage，and at the same time，
the low light tolerance identification was conducted. Beside，this paper analyzed the reduction rate of leaves
chlorophyll content during cucumber seedling stage，and the reduction rate of fruit yield in adult stage，and
also the reduction ratio of plant dry weight. The paper explored the correlation between cucumber tolerance to
low light in seedling stage and adult plant stage. The results showed that the reduction rate of leaves chlorophyll
content during seedling stage under 20 mol·m-2·s-1 light stress at 20 days could be used as indicator for rapid
identification of low light tolerance. The reduction rates of both fruit yield in adult stage and plant dry weight could
reflect the differences in low light tolerance between varieties during adult stage. There existed a significant positive
correlation between leaves chlorophyll content reduction rate in seedling stage and fruit yield reduction rate in adult
stage. Low light tolerance in seedling and adult stages of different varieties was the same. W25 had strong tolerance
to low light，and W5 had poor tolerance to low light.
In order to evaluate the potential breeding value of seleng wormwood（Artemisia selengensis
Turcz.）germplasm resources，this paper carried out identification on the main agronomic traits and nutrition
contents of 14 accessions of seleng wormwood germplasm resources．The results showed that among these 14
accessions，2 mutations A. selengensis var. selengensis and A. selengensis var. shansiensis were included．The
plant height was 31.04-53.38 cm. The internodes of aerial stems were 2.70-4.16 cm in length and 0.51-0.61 cm in
diameter．The tiller numbers of single plant were 6.47-14.01．The yield for aerial stems were 3.01-6.35 kg·m-2 ，
and 0.26-1.08 kg·m-2 for rhizomes．The contents of dry matter，protein，total sugar and crude fiber of aerial
stems were 7.40%-9.60%，1.83%-2.33%，0.46%-0.86% and 0.30%-0.52%，respectively．The accessions
‘Boyanghonggenglouhao-3’ and‘ Poyanghuyehao-4’ were appropriate materials for high aerial stem yield
breeding，and‘ Xiaoyebai’，‘Xiaoyeqing’ and‘ Dayeqing’ were better materials for high rhizome yield
breeding．In addition，the accession‘ Dujiangyanlouhao’ is good for strong aroma breeding because of its special
aromatic ordour．
The effects of cultured trough size（transection width×depth is 20 cm×10 cm，20 cm×15 cm，20
cm×20 cm）and pot volume（diameter 12 cm，height 10 cm，volume 1 L）on plant growth and development，water
and fertilizer uptake and use，yields and quality of elevated cultivation of tomato（Lycopersicon esculentum Mill.）
were studied with the elevated closed-cycle cultivation system．The results showed that compared with pot culture，
trough culture promoted tomato growth，increased production，but the flowering and harvest times were delayed，
the fruit quality and water and nutrients use efficiency were decreased．There were no obvious difference in tomato
yield between different cultured trough sizes，but the plant growth and dry matter accumulation increased，while the
water and nutrients use efficiency and fruit quality were declined．Comprehensively considering the indexes of yield，
quality，water and nutrients use efficiency，20 cm×10 cm was an ideal trough size desired by tomato cultivation．
In order to study the effects of plant growth promoting rhizobacteria HG28-5 on cucumber growth and its rhizosphere microecology，this experiment sowed the cucumber seeds in trays after soaking them in HG28- 5 suspension．Then the quotas of emerging and seedling growth were examined to confirm its promotion effects on cucumber growth．The enzymatic activities in rhizosphere soil，available N，P，K and microbial population were measured to omprehend the effects of HG28-5 on cucumber rhizosphere soil microecology，and to check the colonization densities of HG28-5 in cucumber root and its rhizosphere soil in seedling stage．The results showed that soaking seed in HG28-5 treatment could remarkably increase seedling emergence potential，germination index and improve germination uniformity of cucumber．The soaking treatment with HG28-5 could also remarkably increase the plant height，numbers of leaves，root length，shoot fresh weight and root fresh weight of cucumber during its seedling stage，and significantly improve cucumber root system activity．The soaking treatment with HG28-5 could remarkably improve urease，phosphatase and sucrase activities in cucumber rhizosphere soil，increase the available P content，the amount of bacteria and antinomycetes，While decreaseing the rhizosphere soil fungi quantity during its seeding stage．Besides，HG28-5 could colonize well in cucumber rhizophere．Thirty days after sowing，HG28-5 could maintain a density of 9.20×105 cfu · g-1 and 5.90×105 cfu·g-1，respectively in cucumber root and its rhizosphere soil．
In order to evaluate the characteristics of Phytophthora blight resistance in local pepper resources of Guizhou Province，analyze the correlation between pepper disease resistance and physiological and biochemical indexes，taking 30 Guizhou local pepper resources as experiment material，this paper carries out disease resistance identification through inoculating Phytophthora capsici L. on seedlings，and detects the physiological and biochemical indicators of healthy pepper seedlings．The results showed that there were 1 resistant，9 medium resistant，9 medium susceptible，8 susceptible resources and 3 highly susceptible resources．Analysis showed that pepper seedling disease resistance had significant positive correlation with the soluble sugar content in stem and root， chlorophy content ， vitality of root system，and activities of SOD，POD，CAT，had extremely significant positive correlation with soluble sugar content in leaves，but had significant negative correlation with the content of carotenoid．
In order to clear the control effect of chemical prevention on Tomato chlorosis virus（ ToCV）
disease and sift out chemical pesticides，the control effect of controlling Bemisia tabaci biotype Q by chemical
pesticides were evaluated and the control effect of virus inhibitors on ToCV disease were conducted through
field trial and PCR detection in the lab. The result showed that the viruliferous percent of tomato plants reduced to
3.00%，when Bemisia tabaci biotype Q were killled effectively by insecticide spraying in solar greenhouse. The
control effect of 20% Moroxydine hydrochloride WP，0.5% Portuguese polyene SP，30% Toxic fluoride phosphate
WP to ToCV disease were high as 44.08%-52.63%. The results have provided scientific evidence in chemical
control of ToCV in solar greenhouse.
In order to definit a new disease observed on carrot roots and its pathogen species
of Fusarium causing carrot root rot，this paper isolated the pathogen with tissue isolation method，
inoculated the carrot slices，and carried out pathogenicity identification with morphology and
molecular biology．Pathogenicity was tested with the inoculated carrot slices，inoculated wounded
and non wounded carrots，and carrots planted in pots．The results indicated that 3 strains F1，
F2 and F3 were obtained，and all isolates appeared typical symptoms and showed pathogenicity．
The isolate F1 was identified as Fusarium oxysporum，the F2 and F3 were both identified as Fusarium solani
with morphology preliminary．Sequences of ribosome rDNA-ITS of isolates were tested with primer ITS1/ITS4，
the same results were obtained with morphology，which showed the pathogens were F. oxysporum and F. solani on
carrot root rot.
‘ Tianbai 80’ is a new Chinese cabbage F1 hybrid of mid-late maturity，developed by crossing
male sterile line‘ C441’ with self-incompatible line‘ T01’．Its growth period is about 75 days．The plant is erect
and 51 cm in height，58.5 cm in width．Its outleaf is light green in color with less fuzz and white keel．The head
is of long tube shape and compact in size．It is light green in color．The inside leaf is light yellow in color．Its
head is 46.4 cm in height，11.2 cm in diameter．The head shape index is 4.1．The average single head weight
is about 2.8 kg．It is highly resistant to virus disease and downy mildew，and resistant to black rot．Its average
yield is about 108 t·hm-2．It is suitable to be planted in north and north-west China as mid-late autumn Chinese
cabbage．
‘Jinqie No.1205’ is a new eggplant F1 hybrid developed by crossing inbred lines‘ 338-12-8-5-1’
as female parent and‘ 339-5-9-1-3’as male parent．The plant has strong growth vigor．The first flower sets on
the 7-8th node．The fruit is of round shape with 10.6 cm vertical diameter and 11.6 cm transverse diameter．The
single fruit weight is about 455 g．Its peel is shining black and pulp is tight and light green in color．It has great
taste and good commodity．In fields，its resistance to Verticillium wilt is stronger than that of the contrast．Its
average yield is over 90 t·hm-2．It is suitable for greenhouse cultivation in all parts of Shanxi Province．
‘Kentian No.1’is a new pellicle melon F1 hybrid developed by crossing inbred lines‘ ZT-01-
56’ as female parent and‘ DB-01-38’ as male parent．It is of early maturity with strong fruit setting ability．The
fruit is of oval shape and has yellow green peel．Its pulp is white in color and 1.2 cm in thickness．The single fruit
weight is 350-450 g．The fruit tastes sweet and crispy with a heavy fragrance．The content of central soluble solid
is over 13%．It is resistant to downy mildew and tolerant to powdery mildew．It can yield averagely about 34.5 t·hm-2
．It is suitable for cultivation in protected fields of Heilongjiang，Jilin，Liaoning and Hebei Provinces．
‘Xinnonghuang No.1’ is a new cucumber F1 hybrid developed by crossing inbred lines‘ Y08-2-
5-6-1’ as female parent and‘ Y08-10-1-8-12’ as male parent．The plant grows vigorously and matures early．
It takes 60-65 days from sowing to harvesting．Most of the fruits set on the main stem．The fisrt female flower sets
on about the 5th node，afterwards，every other 2-3 nodes there is a female flower．The fruit is of stick shape，
and shining dark green in color with white thorn on its peel． The whole fruit is 32-35 cm in length and about 3.5
cm in width. Its pulp is light green in color. The single fruit weight is 200-220 g. It can yield over 105 t·hm-2．
It is highly resistant to Fusarium wilt，cucumber angular leaf spot and medium resistant to powdery mildew. It is
suitable for greenhouse， big shed or small tunnel cultivation in protected fields all over Xinjiang.
