ISSN 1000-6346
CN 11-2326/S
中华人民共和国农业农村部 主管
中国农业科学院蔬菜花卉研究所 主办
CN 11-2326/S
中华人民共和国农业农村部 主管
中国农业科学院蔬菜花卉研究所 主办
Taking 142 recombinant inbred lines（‘H3’ב83-60’）F8 group developed by crossing
powdery mildew resistant parental line ‘H3’（Capsicum annuum） and susceptive parental line ‘83-60’
（C.annuum）as studying material，and based on KASPar primer developed by re-sequencing data of parental
lines，this paper constructed pepper genetic linkage map including 16 linkage groups．The total map distance
was 1 356.5 cM，with an average distance of 4.69 cM between adjacent markers．Based on the phenotypic
data of recombinant inbred lines in 2016 and 2017，5 QTLs naming PMR6.1，PMR9.1，PMR11.1，PMR11.2
and PMR5.1 resistant to powdery mildew were detected．Among them，PMR6.1 and PMR9.1 were detected
in these 2 years．The phenotypic contribution rate was between 10.8%-21.4%．This study developed the SNP
molecular marker chain-operated with powdery mildew resistance gene was of important reference value for
pepper disease resistance assistant breeding．
During flowering time process，BrVIN3.1 regulates by mediating the epigenetic states of
BrFLC1，regulated its expression then controlled the flowering time of Chinese cabbage．Taking BrVIN3.1
（Bra020445）as bait，the paper carried out screen selection on yeast cDNA library and obtained BrVIN3.1’s
interaction protein and BrZAT12（Bra006691）．Yeast-two-hybrid verification was conducted．The results
showed that they did interact in vivo．Chinese cabbage materials at different flowering times were treated by
refrigeration．Expression pattern was analyzed．The results showed that BrZAT12 expression patterns were
different in Chinese cabbage materials at different bolting times．The expression quantities of easy bolting
materials were higher than those of tolerant bolting materials，which had lesser amplitude of variation．In
summary，the paper speculated that BrZAT12 may function in flowering pathway by regulating the expression
of BrVIN3.1 in Chinese cabbage．
Taking 6 genetic populations〔P1，P2，F1，F2，B1 （125×F1），B2 （126×F1）〕constructed
by crossing eggplant inbred line 125 and 126 as test material，this paper conducted conjoint analysis of multigenerations
on eggplant plant height trait using major gene and polygene mixed inheritance model．The results
showed that there were significant genetic differences in plant height of tested parent，and the plant height traits
value of segregative generations all showed unimodal partial normal distribution，belonging to quantitative
inheritance．The results also showed that the most suitable genetic model for plant height trait was C-0 genetic
model．There was no major gene genetic effect existed，showing a genetic model controled by polygene with
additive-dominance-epitasis．The polygene genetic effects of plant height was further analyzed according to
the second order genetic parameters．The results showed that the polygene heritability in F2 and B2 eggplant
segregative generations were 49.24% and 22.77%，respectively．The plant height of eggplant gave priority
polygene inheritance．
Taking moderately stronger flood tolerant melon ‘Cuixi’ and relatively weaker flood tolerant
melon‘ Shijimi’ as materials，this paper studied the effects of half submerged and total submerged（1，3 days）
stress and stress relieve（1，3，5 days）on respiration and other physiological metabolism of melon seedlings．
The results indicated that comparing with the contrast carrying out normal water management，waterlogging
stress decreased the fresh weight，dry weight and activities of melon seedlings root system，and the inhibiting
effect of total submerge treatment was more significant．Waterlogging stress increased the activities of lactate
dehydrogenase（LDH）and alcohol dehydrogenase（ADH），reduced the activities of malate dehydrogenase
（MDH）and succinate dehydrogenase（SDH）．The reducing scale of ‘Shijimi’ with relatively weaker flood
tolerance was larger than that of ‘Cuixi’ with moderately stronger flood tolerance．The activities of superoxid
dismutase（SOD），peroxidase（POD）and catalase（CAT）of these 2 varieties showed an increasing tendency
after waterlogging stress．Therefore，waterlogging stress inhibited the aerobic respiration of melon seedlings，
while accelerated the anaerobic respiration metabolism，eventually leading to restrain the root system growth．
Moreover，the inhibition rate suffered by‘ Shijimi’ was larger than that of‘ Cuixi’．
Taking ‘Xianjiaowang’ pepper as test material and by potted culture method，this paper
explored the main root system in phosphorus（P）high efficient utilization and rhizosphere process through
analyzing the changes in mophology and physiology of pepper root system under different phosphorus
application levels．The results showed that the biomass of pepper overground part and root system increased
firstly then decreased with the increase of P application．Along with the increase of P supply，the total root
length showed a tendency of first increase then decrease，and the average root diameter and ratio of root to
shoot decreased gradually．The rate of arbuscular mycorrhizal colonization and activity of soil acid phosphatase
in pepper root system increased firstly then decreased．The correlation analysis showed that soil Olsen-P
concentration and whole plant P concentration were extreme significant negatively correlated with average root
system diameter，and root arbuscular mycorrhizal colonization；and could be fitting with the total root length
by quadratic equation with one unknown，indicating that the rhizosphere trait of pepper root system adapted to
low P might be achieved by increasing root biomass and total root length，while reducing the average diameter
of root system．
Aiming at the issues of serious occurrence of potato fungal diseases，mixed occurrence of
various diseases，lacking effective control methods，this paper classified and identified highly effective
antagonistic strain BA-26 screened out at earlier stage，studied the stability of its extracellular metabolites，
and clarified its role on preventing disease and promoting growth．BA-26 strain was identified as Bacillus
amyloliquefaciens，through analyzing morphologic characteristics，physiological and biochemical properties，
and 16S rDNA sequence analysis．This strain showed strong antagonistic effects on 8 potato pathogenic
fungus，and its inhibition ratio against Alternaria solani，Fusarium solani and Fusarium avenaceum was
over 80%．After treating the extracellular metabolites by 100 ℃ for 30 min，UV irradiation for 8 hours，pH
2-10 acid-base，and protease for an hour，its relative antibacterial activities were all maintained above 80%．
Laboratory test of bacteriostatic activity showed that the inhibition ratio of BA-26 filtrate against Fusarium
solani was 91.3%．In pot experiment，the potato root length，fibrous root number，plant height and
chlorophyll content in leaves were significantly increased by 32.07%，111.94%，48.0% and 19.6%，
respectively by applying BA-26 fermentation broth with 20 mL concentration of 4.0×109 cfu·mL-1 per pot．
The results indicated that B. amyloliquefaciens BA-26 had broad bacteriostasis spectrum，strong stability of
extracellular metabolites，and good disease prevention and growth promotion effect，having great potential for
developing biocontrol agent exclusively for potato．
It is of important significance to ensure the stable supply of vegetables．In recent years，under
the contrasting function of the benefits from non-agricultural industries，the willingness of farmer households
for planting vegetables is related to the development of vegetable industry．On the basis of livelihood capital
frame，this paper analyzed the characteristics of farmer households financial capital by Logit model，and
conducted empirical analysis on how financial capital affecting farmer households willingness for vegetables
cultivation．The main conclusion is that financial capital is an important livelihood component of farmer
households，of which nearly 95% is at the middle and lower levels．Besides，farmer households income level
and government subsidies have a positive impact on their willingness for sustainable vegetables cultivation．
While，obtaining loan just has a negative effect，and insurance has not significant effect．Before ending，the
paper puts forward corresponding countermeasures and suggestions．
Taking field investigation combined with questionnaire survey，this paper investigated and
analyzed the usage condition of fertilizers and pesticides in 5 main watermelon and melon production areas in
Hebei Province．The results showed that excessive application of fertilizers and pesticides，big production
input in watermelon and melon production was ubiquitous phenomenon．Over 30 kinds of pesticides were
applied．The pure nutrient content of converted nitrogen，phosphorus and potassium of fertilizer during the
whole growth period was 25，15，30 kg · （667 m2） -1，respectively．The paper suggested to popularize the
following techniques：integration of water and fertilizer，sealing shed with high-temperature，raising seedlings
by grafting，adopting honeybee pollination，etc.，so as to form a standard and efficient planting mode with
reduced fertilizer and pesticide application，and improve the use ratio of fertilizer and pesticide．
Potato production area and total yield in Inner Mongolia ranks front in China，but its per
unit area yield is below the average national level．Unreasonable nutrients management is an important factor
restricting potato yield．In order to find out the problems existing in irrigated potato nutrient inputs，this paper
conducted peasant household investigation in 9 main potato producing counties during 2013-2016 in Inner
Mongolia．The results indicated that the input of nitrogen fertilizer was exorbitant，and over-input or shortage
of phosphorus and potassium fertilizer were coexistent．Nitrogen partial factor productivity of 57.3% peasant
households was lower than 100 kg · kg-1 for irrigated potato production in Inner Mongolia，having bigger room
for improvement．There existed remarkable correlativity between planting scale of peasant households，yield
level of potato，nitrogen fertilizer application amount，and nitrogen partial factor productivity．
‘Yulian No.1’ is a straight tube mid-late maturing Chinese cabbage F1 hybrid bred from crossing
self-incompatible line ‘Yutianbaojian S05-8’ as female parent and self-incompatible line ‘Lianshu S05-23’
as male parent．Its growth period is 80-85 days．The plant is of straight tube type with green/white band and
dark green leaf．The plant height is about 48 cm with about 62 cm stretch．The leaves are shining bright with
little wrinkle，and outer leaves are erect．The head center leaves are yellow-green in color．Its head is 43 cm
in height，and 15 cm in diameter．The single head weight is 2.4 kg．It can yield 132 t·hm-2．It is tolerant to
storage．The head is soft with good flavor and quality．In the fields，its resistances to virus disease，downy
mildew，soft rot and tip burn are better than that of the contrast ‘Jinlyu 75’．It is suitable for planting in
Liaoning，Jilin Provinces and similar ecoregions．
‘Jingyanzhoupi No.1’ is a new early maturity hot pepper F1 hybrid developed by crossing inbred
line‘ M1-1’ as female parent and inbred line‘ F24B-1’ as male parent．The initial flower is setting on the 7-8th
node．It takes about 38 days from planting to harvesting．Its plant has medium growth vigor and strong ability
for continuous fruit setting．The fruit expands fast with little differences between top one and bottom one．It
has good commodity．There are folds and twists on its peel．The immature fruit is light green in color，and
mature one is red in color．It is about 6.8 cm in length，5.0 cm in width，and 0.25 cm in thickness．The single
fruit weight is about 40 g．Its outstanding features are thin peel，crisp and tasty，with moderate piquancy，
and high acceptance by consumer．In fields，its resistances to virus disease，pepper blight and anthracnose are
stronger than that of the contrast ‘Zhoupila’．It can yield about 50.7 t · hm-2．It is suitable for cultivation in
protected and open fields in Yunnan Province．
‘Zaoguansigua 408’ is a loofah gourd F1 hybrid developed by crossing ‘S4’ as female parent
with‘ X08’ as male parent．This variety is of extremely early maturity．It takes about 60-62 days from sowing
to harvesting．The plant is rampant with strong growth potential and branching ability．The first female flower
grows on the 5-8 knob of the main vine．The fruit is of long cylindrical shape，the apex is round，and the peel
is green in color．Its single fruit weight is 0.4-0.8 kg．The fruit is 40-49 cm in longitudinal diameter and 3.8-
4.1 cm in transverse diameter．It can yield 16.5 t · hm-2 at earlier stage and its yield of whole stages can reach
48.0 t · hm-2．Its resistance to powdery mildew，downy mildew，and Phytophthora blight are better than that
of the contrast‘ Yahuazaosigua’．It is suitable for cultivation in early spring greenhouses and open fields in the
Yangtze River Basin and Hunan Province．
‘Shifan No.45’ is a F1 hybrid of processing tomato developed by crossing ‘W17-3’ as female
parent and‘ K88-10’ as male parent．It is of limited growth type．The plant has strong trunk and strong growth
vigor．The average plant height is 70 cm without node in fruit stalk．The fruit is easy to separate from sepals
when be shaken during picking．The fruit is of oval shape and red in color．The single fruit weight is 58 g．
The soluble solids content is 5.1%．The pH value is 4.32，and the content of lycopene is 119.2 mg · kg-1．
The fruit number per plant can be up to 60．Its fruit is resistant to cracking，tolerant to pressure，storage and
transportation．Single fruit compression resistance can be 6 kg．It takes about 125 days from sowing to 85%
red fruit ripening ready for machine-picking．In fields，its resistance to early blight is stronger than that of the
contrast ‘H3402’．It is suitable for planting and mechanical harvesting in processing tomato planting areas in
Southern and Northern Xinjiang．The yield can reach above 124.5 t·hm-2．
