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Current Issue
2021 Vol.1 Issue.1,
Published 2021-01-01
1
Hot!
2021 Vol. 1 (1): 1-3 [
Abstract
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194
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2280KB] (
1149
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4
Hot!
2021 Vol. 1 (1): 4-10 [
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268
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1448KB] (
1823
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11
2021 Vol. 1 (1): 11-14 [
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182
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15
Research Progress on Cabbage Genetic Breeding During ‘The Thirteenth Five-year Plan’ in China
YANG Limei,FANG Zhiyuan,ZHANG Yangyong,ZHUANG Mu,LYU Honghao,WANG Yong,JI Jialei,LIU Yumei,LI Zhansheng,HAN Fengqing
Hot!
During‘The Thirteenth Five-year Plan’period,important progress on cabbage genetic
breeding in China was achieved.A number of new and novel germplasm resources was created.Some genes/
QTLs associated with important agronomic traits were mapped or cloned.A batch of new cabbage varieties with
good quality and multiple resistances was developed.This paper summarized the important research progress
in recent 5 years from the aspects of germplasm innovation,mapping/cloning of genes/QTLs with important
agronomic traits,and breeding of new varieties,etc.The paper also analyzed the main problems existing in
present cabbage breeding and the development direction in future.
2021 Vol. 1 (1): 15-21 [
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250
) [
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1KB] [
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874KB] (
2023
)
22
Research Progress on Chinese Cabbage Genetic Breeding During‘The Thirteenth Five-year Plan’in China
ZHANG Fenglan,YU Shuancang,YU Yangjun,ZHANG Deshuang,SU Tongbing,ZHAO Xiuyun,WANG Weihong,LI Peirong,XIN Xiaoyun
Hot!
During‘The Thirteenth Five-year Plan’period,significant progress has been achieved on
genetic breeding of Chinese cabbage〔
Brassica campestris
L. ssp.
pekinensis
(Lour)Olsson〕in China.
A number of novel breeding materials with outstanding resistance to new epidemic diseases and composite
resistance has been obtained by widely applying techniques combining conventional breeding,markerassisted
breeding DH and high throughput MAS breeding in cabbage breeding programs.A batch of new
Chinese cabbage varieties with high taste quality and diversified product types has been released and applied
in production.In addition,the basic research for application in China has been in the international leading
level.This paper systematically summarized the important progress obtained on applied basic research,
breeding technique research,germplasm innovation and new variety selective breeding of Chinese cabbage
during 2016-2020.The paper also discussed and analyzed the changes in industry,existing problems and the
development direction in future.
2021 Vol. 1 (1): 22-32 [
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246
) [
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1KB] [
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1014KB] (
1935
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33
Research Progress on Broccoli Genetic Breeding During ‘The Thirteenth Five-year Plan’ in China
LI Zhansheng,LIU Yumei,HAN Fengqing,FANG Zhiyuan,ZHANG Yangyong,YANG Limei,ZHUANG Mu,LYU Honghao,WANG Yong,JI Jialei
Hot!
During ‘The Thirteenth Five-year Plan’,the genetic breeding of broccoli in China has
been quickly developed.A batch of broccoli new varieties and breeding resources with excellent traits has
been selected and bred.The bottleneck problem in seed production based on male sterility has been broken
through.So far,the market-share of broccoli new cultivars bred in China has grown up to 15.36% from
insufficient 5% in 2010.At the same time,the applying basic research in China developed rapidly,our genetic
breeding technology of broccoli has reached the international advanced level.This paper reviewed the research
achievement and progress made in China since 2016 from the aspects of broccoli germplasm innovation,new
cultivars selective breeding,breeding technologies,etc.The paper also put forward the existing potential
problems and future development trend for broccoli industry.
2021 Vol. 1 (1): 33-40 [
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] (
311
) [
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889KB] (
2201
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41
Molecular Marker-assisted Selective Breeding of Sweet Pepper Inbred Line with Resistance Gene(
L
4
)to Tobamovirus and Resistance Gene(
Pvr4
)to Potato virus Y
YU Hailong,WANG Lihao,ZHANG Baoxi,ZHANG Zhenghai,CAO Yacong
Hot!
Tobamovirus
is one of the most important viruses harming pepper production in China.In
recent years,damages by pepper mild mottle virus(PMMoV) has become more and more serious.The
L
allelic genes(
L
1
,L
2
,L
3
,L
4
) are the main resistant genes providing resistance against
Tobamovirus
.In order
to improve the
Tobamovirus
and PVY resistance of pepper varieties,this experiment took‘ CM334’( with
resistance to PVY,containing
Pvr4
gene)and imported material ‘200375’(with resistantce to PMMoV,
containing
L
4
gene)as resistant resources,and sweet pepper inbred line‘ 83-163’( medium resistance
to Phytophthora blight and CMV)as backcross parents,and conducted multi-generation backcross and
selfing.Utilizing molecular marker-assisted selection(MAS)combined with seedling resistance identification
and morphological selection,this experiment at last obtained the early-maturing,large fruit sweet pepper
selfing line ‘PT83-163’,polymerizing 2 resistance genes
L
4
and
Pvr4
,both resistant to
Tobamovirus
and
PVY(medium resistant to epidemic disease and CMV,containing resistant gene
Pvr4
and
L
4
).By artificial
inoculation identification,line‘ PT83-163’ was resistance to disease and its horticultural traits were comparable
to that of the inbred line‘83-163’.
2021 Vol. 1 (1): 41-47 [
Abstract
] (
218
) [
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1909KB] (
689
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48
Effects of Exogenous 24-epibrassinolide on Tomato Seedling Growth and Potassium Accumulation Under Sub-low Temperature
YANG Lian,GAO Huan,WU Fengzhi
Taking 2 tomato varieties‘Dongnong 708’(‘DN 708’) sensitive to low temperature and
‘Dongnong 722’(‘DN 722’)tolerant to low temperature as test materials,this paper studied the effects
of 24-epibrassinolide(EBR)on tomato growth and potassium accumulation under sub-low temperature
condition.The results showed that comparing with the suitable temperature,low temperature stress could
inhibit the seedling growth of these 2 tomato varieties,and remarkably reduce the maximum photochemical
efficiency of PSII (F
v
/F
m
),and K
+
concentrations in stems and leaves,and significantly increase the
malondialdehyde(MDA)content and electrolyte permeability.Moreover,the effects of sub-low temperature
stress on‘DN 708’was greater than that on‘DN 722’.While,exogenous EBR effectively alleviated
the inhibition of sub-low temperature stress on tomato seedling growth,significantly improved F
v
/F
m
,K
+
concentrations in roots,stems and leaves of these 2 tomato varieties,and increased relative expression levels of
LKT1,SIHAK5
and
SIHAK9
genes in roots and leaves,and significantly reduced MDA content and electrolyte
permeability in seedlings of these 2 tomato varieties.And the variation range in‘DN 708’was greater
than that in‘DN 722’.To sum up,exogenous EBR may increase the K
+
concentration in various organs of
tomato seedlings by promoting the expression of transport related genes and K+ absorption,reduce the degree
of cell membrane peroxidation,and enhance the adaptability of tomato seedlings to sub-low temperatures
condition.Thus,exogenous EBR has a greater mitigation effect on low temperature sensitive tomato variety‘ DN
708’ under sub-low temperature stress than low temperature tolerant tomato variety‘ DN 722’.
2021 Vol. 1 (1): 48-55 [
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207
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1305KB] (
587
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56
Comprehensive Evaluation on Bolting Nature of Welsh Onion Germplasm Resources
YANG Guodong,LI Na,JIA Junxiang,TIAN Yun,JIANG Xintao,JIANG Binbin,CUI Lianwei
Taking 28 different types germplasm resources of welsh onion as research objects,this paper
selected 9 major traits at bolting and flowering stages of welsh onion as evaluation indexes on bolting nature,
and conducted comprehensive evaluation on welsh onion bolting nature by principal component clustering
analysis,average membership degree and bolting index methods.The results showed that the factors with
bigger contribution to principal component of welsh onion bolting nature were initial bolting date,early
flowering date,number of leaves and bolting speed.Their cumulative contribution rate reached 58.93%.
Specially,initial bolting date had higher correlation with the other indexes,which could be the leading
indicators for bolting nature evaluation.The bolting nature of different welsh onion germplasm resources could
be distinguished effectively by principal component clustering analysis,average membership degree and bolting
index methods.Among them,the average membership degree and bolting index methods could provide more
visual and clear partition on bolting nature of welsh onion germplasm resources.These 2 methods had similar
results.But the former’s merit was comprehensive,and the latter’s merit was simple and convenient.
2021 Vol. 1 (1): 56-61 [
Abstract
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194
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866KB] (
609
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62
Effect of Different Full Biodegradation Film on Stem Lettuce Production
LI Shuifeng,WANG Huaying,XU Chuchu,FAN Zhengwei,ZHONG Shuiliang,HU Meihua
Ten kinds of full biodegradation films with different specifications were introduced and tested
on stem lettuce production.Their effects on yield were studied.The results showed that except ‘Shanghai
black 6’,the other degradable films had no significant effect on stem lettuce yield comparing with the ordinary
polyethylene film.But there was the tendency of yield decreasing along with the decrease of film thickness.
The thinner the film thickness was,the faster the degradation rate was.The time duration for black degradation
film entering into induction period was generally earlier than that of the white one.Comprehensively seen from
film degradation status and stem lettuce yield,full biodegradation film‘ Yunnan white 10’ was the most suitable
film mulching for stem lettuce cultivation in early spring.
2021 Vol. 1 (1): 62-65 [
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199
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1KB] [
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874KB] (
590
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66
Screening and Identification of Phosphate-solubilizing Microorganism(PSM)in Burdock Rhizosphere Soil and Optimization of Phosphate-solubilizing Conditions
SUN Ke,GENG Fengying,YU Qiuju,WANG Feng
Taking phosphate-solubilizing circle method and soluble phosphorus content determination
method,this experiment screened out PSM-5 strain with stronger phosphate-solubilizing ability from
Arctium
lappa
L. rhizosphere soil and optimized the phosphate-solubilizing conditions on screened strains.The results
indicated that the strain PSM-5 was tentatively identified as Bacillus subtilis,and after optimization the optimal
conditions for phosphate solubilization were initial 3.79 pH value,28.46 ℃ fermentation temperature and 10.46
g · L
-1
salinity.These results have provided phosphate-solubilizing bactericide for next step development of
bio-organic fertilizer dedicated for
Arctium lappa
L.
2021 Vol. 1 (1): 66-72 [
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181
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1761KB] (
569
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73
Effect of Different Numbers of Vernalization Day on Autumn Generation-adding Seed Reproduction of Pakchoi in Southern Zhejiang
SHU Jun,HUANG Wenbin,LOU Jianying,FENG Guang,ZHANG Jianlong,LI Lebin,ZHANG Jingli,WU Xiaobao
Taking 3 types of pakchoi〔
Brassica campestris
L. ssp.
chinensis
(L.)Makino var.
communis
Tsen et Lee〕with different bolting ability as test material,this experiment adopted different low temperature
and illumination to induce vernalization on seedlings,and studied the effects of 9 vernalization combinations
on vegetative mass,bolting,blooming and seed quality of pakchoi.The results showed that the maximum
differences in plant open width degree among different vernalization combinations in early,medium and late
bolting types were 17.1,6.3 and 6.5 times.And the maximum differences in single plant weight were 5.6,
2.8 and 2.9 times,respectively.The flowering date of each bolting type were significantly ahead of schedule,
along with the increase of vernalization days number.For the early bolting,medium bolting and late bolting
types,the maximum differences of flowering date between the different vernalization combinations of early
bolting and medium bolting types were 54,39 and 36 days.For the early bolting and medium bolting types,
the average single plant seed weight and germination rates were the best,when the vernalization treatments
were happened on 20-20,20-25,respectively.By adjusting bolting anthesis and seed collecting stage,
breeding generation-adding link and normal breeding cycle were complete articulation,thus realizing the
breeding target of 2 generations one year for pakchoi in southern Zhejiang areas.
2021 Vol. 1 (1): 73-78 [
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171
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1010KB] (
613
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79
Residues and Digestion Dynamics of Cyantraniliprole and Pymetrozine in Pumpkin
LI Anying,ZHANG Shaojun,CHEN Yongda,ZHAO Xudong,LI Hongyan,ZHENG Zhenshan
This paper developed an analytical method for detection of cyantraniliprole and pymetrozine
residues in pumpkin,and studied the digestion dynamic rule and final residues of cyantraniliprole·pymetrozine
water dispersible granules in pumpkin from Hebei and Zhejiang Provinces.The samples were extracted
with acetonitrile,purified by N-propyl ethylenediamine(PSA)and determined by liquid chromatography
tandem mass spectrometry.The results showed that the limit of quantification( LOQ) of cyantraniliprole
and pymetrozine in pumpkin samples were 0.01 mg · kg
-1
and 0.005 mg · kg
-1
.The addition recovery
rate of cyantraniliprole in the range of 0.01-1.0 mg · kg
-1
was 100%-104%.The addition recovery rate of
pymetrozine in the range of 0.005-0.5 mg·kg
-1
was 96%-107%.The residue digestion rules of cyantraniliprole
and pymetrozine in pumpkin were all conformed to the first-order kinetics process.The final residue of
cyantraniliprole in pumpkin was ≤ 0.031 mg · kg
-1
,and the final residue of pymetrozine was ≤ 0.023 mg ·
kg
-1
.The recommended dosage by enterprise was 450 and 675 g·hm
-2
,and the safe interval was 14 days.
Pumpkin fruits during harvesting period were safe to eat.
2021 Vol. 1 (1): 79-83 [
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262
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1163KB] (
439
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84
Biological Control Experiment on Leek Gray Mould in Protected Fields
WANG Dan,SHI Zhaopeng,LI Ping,LIU Cunhui,YIN Shanshan, LI Jishun,ZUO Libo,SUN Zuowen
Grey mold is an important disease for leek cultivation in protected fields.Five Trichoderma
strains were selected and sprayed on leek seedling and field control experiment was carried out on leek gray
mold.The results showed that the control effects of
Trichoderma atroviride
20111,
T. harzianum
C61,
T.
harzianum
LTR-2,
T. harzianum
21990 and
T. atroviride
23617 were 13.62%,68.93%,64.00%,91.72%
and 87.23%,respectively.Among them,
T. harzianum
21990 had the best control effect.On this basis,
a cooperative control test on
Trichoderma
sp. and
Bacillus subtilis
was carried out.The results indicated that
spraying
T. harzianum
21990 mixed with water before covering the leek planted shelter,then spraying
Bacillus
subtilis
when the leek grew up to about 5 cm in height,the field control effect could reach 74.07%.
2021 Vol. 1 (1): 84-88 [
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208
) [
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1KB] [
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902KB] (
792
)
89
A New
Luffa acutangula
F
1
Hybrid —‘Guiguan No.5’
KANG Dexian,LI Yian,LU Fashi,WU Yongguan,LU Yacheng,JIANG Yaqin,MO Yangpeng,GAN Guiyun1,LI Weiliu1,WANG Yikui,LI Wenjia1
‘Guiguan No.5’is a F1 hybrid of
Luffa angustifolia
developed by crossing inbred line‘B05-
13-2’as female parent and inbred line‘A10-12-3-7’as male parent.The plant has strong growth vigor and
strong branching ability.Both its main and lateral vines can bear melon.It is of early maturity.The first
female flower sets on the 12-13th node in spring,but on 16-18th node in autumn.It takes 65-68 days from
sowing to first harvest in spring and 45-47 days in autumn.The commercial melon is of short stick shape.Its
rind is green with spots on the surface.The melon is 35-38 cm in length,5.0-5.2 cm in diameter.The single
fruit weight is 310-350 g.Its flesh is white in color and tastes tender and sweet.The yield is 52.5-58.5 t · hm
-2
.
Its resistances to downy mildew,powdery mildew and bacterial angular spot in fields are stronger than that of
the contrast‘Guilinbalingua’ .It is suitable for planting in spring,summer and autumn in Southern China.
2021 Vol. 1 (1): 89-91 [
Abstract
] (
186
) [
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1KB] [
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2738KB] (
551
)
92
A New Pickled Pepper F
1
Hybrid —‘Qianla No.10’
YANG Hong,JIANG Hong,LIU Chongzheng,LAI Wei,YAN Xi
‘Qianla No.10’is a new pickled pepper F
1
hybrid developed by crossing cytoplasm male sterile
line‘8719A’as female parent and inbred line‘87-22-3C’as male parent.It is of early maturity,and
takes about 170 days for whole growth period.The plant has strong growth vigor and continuous fruit setting
ability.Its fruit grows upward,and is of orbicular.The fruit is about 2.0 cm in length,and about 2.9 cm in
width.The average single fruit weight is 11.2 g.Its VC content is 1 505 mg · kg
-1
.The capsaicin content is
114 mg · kg
-1
.In fields,its resistance to anthracnose is stronger than that of the contrast‘Meijiangmingzhu’.
This variety can yield about 22.5 t · hm
-2
fresh pepper.It is suitable to be planted in Zunyi,Bijie,Anshun,
Guiyang,and states of Qiannan,Qianxi’nan of Yunnan-Kweichow plateau areas in spring.
2021 Vol. 1 (1): 92-95 [
Abstract
] (
187
) [
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1KB] [
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2740KB] (
655
)
95
A High Quality and Cracking Tolerant New Watermelon F
1
Hybrid —‘Jingying’
REN Yi,GONG Guoyi,ZHANG Haiying,GUO Shaogui,ZHANG Jie,LI Maoying,YU Yongtao,XU Yong
‘Jingying’is a new early maturity,high quality,tolerance to cracking and long-time
storage mini-watermelon F
1
hybrid, developed by crossing selfing line‘XM’as female parent and selfing
line‘HX3’as male parent.The plant has strong growth vigor and is easily bearing fruit under low temperature
after grafting.It takes about 90 days for whole growth period and 30-35 days for fruit development stage.
Its fruit is of elliptical shape with dark green rind,narrow stripes on the green peel.The single fruit weight
is about 2.0 kg.Its flesh is even red in color.The central sugar content is 12%-13%,the highest one can
reach 15.7%.The pericarp is thin and tenacious.The flesh is anti-cracking and anti-dehydration,crisp with
good taste and long-time storage tolerance.Its shelf life can reach 15 days.The yield is about 52.5 t · hm
-2
.
It is suitable for high-quality cultivation in protected fields all over China and for long-distance transportation.
2021 Vol. 1 (1): 95-97 [
Abstract
] (
322
) [
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1KB] [
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2711KB] (
450
)
98
A New Black Waxy Corn F
1
Hybrid —‘Jinnuo No.20 ’
FAN Rui,CHEN Yongxin,DONG Lihong,ZHAI Guangqian,LI Wenhe,RUAN Fulin
‘Jinnuo No.20’is a new black waxy corn F
1
hybrid developed by crossing inbred line‘Tian
BN’as female parent and inbred line‘chN1’as male parent.Its ear is of cylindrical shape.The maize
cob and kernels are purple black in color.In northern production areas,its growth period is about 91 days.
The plant height is 279 cm,ear position height is 127 cm.Its ear is 20.5 cm in length,4.8 cm in thickness.
There are 16 head progeny rows.The amylopectin holds 99.09% of the total starch content.The fresh ear yield
is about 14.1 t · hm
-2
.It is medium resistant to leaf blight and gall smut.In the southern production areas,
its growth period is about 76 days.The plant height is 225 cm and ear position height is 86 cm.Its ear is
17.2 cm in length,4.9 cm in thickness.There are 14-16 head progeny rows.The amylopectin holds 98.30%
of the total starch content.The fresh ear yield is about 12.0 t · hm
-2
.It is medium resistant to corn sheath
blight.It is suitable for planting in the northern regions of Jilin,Inner Mongolia,Hebei,Shanxi,etc.,and
in the southern regions of Zhejiang,Jiangxi,Fujian,Hainan,etc.
2021 Vol. 1 (1): 98-101 [
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] (
249
) [
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1KB] [
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2669KB] (
449
)
102
2021 Vol. 1 (1): 102-104 [
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] (
181
) [
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1KB] [
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2366KB] (
580
)
105
2021 Vol. 1 (1): 105-107 [
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] (
169
) [
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1KB] [
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1705KB] (
424
)
108
2021 Vol. 1 (1): 108-109 [
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] (
199
) [
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1KB] [
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1439KB] (
488
)
110
2021 Vol. 1 (1): 110-112 [
Abstract
] (
169
) [
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1KB] [
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1007KB] (
633
)
112
2021 Vol. 1 (1): 112-116 [
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] (
181
) [
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1KB] [
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2224KB] (
592
)
117
2021 Vol. 1 (1): 117-122 [
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] (
177
) [
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1KB] [
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1412KB] (
498
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