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  Current Issue
 
2021 Vol.1 Issue.4,Published 2021-04-01
1
Hot!
2021 Vol. 1 (4): 1-4 [Abstract] ( 193 ) [HTML 1KB] [ PDF 1356KB] ( 866 )
5
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2021 Vol. 1 (4): 5-11 [Abstract] ( 177 ) [HTML 1KB] [ PDF 1696KB] ( 810 )
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2021 Vol. 1 (4): 12-15 [Abstract] ( 186 ) [HTML 1KB] [ PDF 916KB] ( 563 )
16 Research Progress on Cucumber Genetic Breeding During ‘The Thirteenth Five-year Plan’ in China
ZHANG Shengping，MIAO Han，BO Kailiang，DONG Shaoyun，GU Xingfang Hot!
During the “Thirteenth Five-year Plan”period，cucumber basic research for application
developed rapidly in China，molecular breeding technology advanced continuously，gene editing technology
has achieved break through，and new varieties have been upgraded．This paper summarized the latest
progress made in the past 5 years from the aspects of activating research on cucumber gene with important
agronomic traits and function，molecular marker technology，haplotype breeding，transgenic and gene editing
technology，germplasm resource evaluation and screening, breeding material innovation，and new variety
selective breeding，etc．The paper also analyzed the existing problems and prospected the future works．
2021 Vol. 1 (4): 16-26 [Abstract] ( 291 ) [HTML 1KB] [ PDF 921KB] ( 1288 )
27 Research Progress on Heat Stress and Heat Tolerance in Solanaceous Vegetables
YU Chuying，YIN Yanxu，Wang Fei，LI Ning，GAO Shenghua，Juntawong Niran，WU Jun，JIAO Chunhai，YAO Minghua Hot!
Solanaceous vegetables are one of the most important fruits and vegetables in vegetable production
in China．However，extreme weather such as high temperature etc. has seriously restricted their growth and
development，damaged their yield and quality．Therefore，it is particularly urgent to carry out studies on heat
tolerance of solanaceous vegetables．This paper reviewed the effects of heat stress on growth，development and
physiological metabolism of solanaceous vegetables；research progress made on identification of heat tolerance，
mechanism of heat stress response and heat tolerance，and measures to improve heat tolerance，etc．The paper
also prospected the future research direction，aiming at providing methods and ideas for further analysis of heat
tolerance mechanism，and improving studies on heat tolerance of solanaceous vegetables．
2021 Vol. 1 (4): 27-40 [Abstract] ( 270 ) [HTML 1KB] [ PDF 1385KB] ( 899 )
41 Application of Genome-wide Association Study in Vegetable Breeding
ZENG Meijuan，LIU Jianting，ZHUO Lingling，CHEN Mindong，YE Xinru，WANG Bin，ZHU Haisheng*，WEN Qingfang
Genome-wide association study（GWAS）is a method efficiently associating phenotype with
genotype，and being used in genetic mapping and searching for candidate genes with related traits．It can be
used to carry out associated analysis on multiple complex traits at the same time，and is widely used in vegetable
breeding．This paper reviewed the method of whole genome association analysis；summarized its application
recently in studying the related traits，including quality，yield and resistance during vegetable growth and
development process．The paper also looked forward to its application prospect in vegetable breeding．
2021 Vol. 1 (4): 41-47 [Abstract] ( 193 ) [HTML 1KB] [ PDF 947KB] ( 670 )
53 Morphological Characteristic Changes in Floral Bud Differentiation Process of Welsh Onion
YANG Guodong，LI Na，JIA Junxiang，Tian Yun，JIANG Xintao，CUI Lianwei
In order to clarify morphological characteristic in the floral bud differentiation process of Welsh
onion（Allium fistulosum L. var. giganteum Makino），this study took easy bolting variety‘Liaocong No.5’ and
tolerant bolting variety ‘Tianguangyiben’ as test material；conducted artificial low-temperature vernalization
at seedling stage；observed the floral differentiation process and changes of morphological characteristic in
green Welsh onion by anatomical lens；and divided the period of floral bud differentiation．The results showed
that the floral bud differentiation process and changes in morphological characteristic of these 2 Welsh onion
varieties with different bolting ways were basically the same．They could be classified into 4 phases including
induction stage，initial stage of flower bud differentiation，inflorescence and flower primordium differentiation
stage，flower organ differentiation stage．Plant transformed from nutrional growth to reproductive growth
when vegetative growth cone turned into a central convex ring form，might being considered as the initial
sign of the floral bud differentiation of Welsh onion．The closure or impending closure of the involucral
bracts was considered as signs for beginning of the inflorescence and flower primordium differentiation
stage．When the flower stem extended to 5-10 mm，an oblate outer peripheral primordium and a spherical
outer stamen primordium formed at the apex of each triangle flower primordium on the upper part of the
inflorescence axis，marking the initiation of flower organ differentiation．Floral organ differentiation entered
into late stage when 30% top florets opened after involucral cracking．There were larger differences between the
induction durations of‘Liaocong No.5 ’and ‘Tianguangyiben’，by 20 days and 35 days，respectively．The
duration of initial flower bud differentiation，inflorescence primordia and floral primordia differentiation，floral
organ differentiation were similar，being 10 days，15 days，and 30 days，respectively．
2021 Vol. 1 (4): 53-58 [Abstract] ( 261 ) [HTML 1KB] [ PDF 1412KB] ( 768 )
59 Establishment of a Regeneration System in Wax Gourd（ Benincasa hispida Cogn.）
LI Zhifang，YAN Jinqiang，HAN Xiangfeng，XIE Dasen，LIU Wenrui，WU Qianhui，JIANG Biao
In this study，taking cotyledon nodes as explants，the effects of different proportions and
concentrations of hormones and days of dark culture on cotyledon node callus-induced regeneration buds were
optimized，the effect of different concentrations of hormone combinations on adventitious bud proliferation
were studied，and the rooting and transplanting of regenerated seedlings were tested，finally we established
a relatively comprehensive set of regeneration system of wax gourd cotyledon nodes．According to the result
of orthogonal experiment，the optimum combination for inducing adventitious bud differentiation was MS +
2，4-D 0.1 mg · L-1 + IBA 0.10 mg · L-1 + 6-BA 0.8 mg · L-1 after 7 days dark culture；and the time of dark
culture and concentration of 2，4-D were major factors affecting regeneration．The best medium for proliferation
of adventitious bud was MS + NAA 0.05 mg · L-1 + 6-BA 0.2 mg · L-1，with a proliferation coefficient of 2.487．
The rooting rate of the regenerated seedlings on the rooting medium was 100% on 1/2MS + NAA 0.2 mg · L-1
and the survival rate of the domesticated seedlings was more than 80% after transplanting．
2021 Vol. 1 (4): 59-63 [Abstract] ( 228 ) [HTML 1KB] [ PDF 1313KB] ( 803 )
64 Establishment of Identification Method for Resistance to Bacterial Angular Leaf Spot in Chinese Cabbage
REN Junhui，LIU Yan，JI Jingjing，CHEN Zhiqiang，ZHANG Yaowei
Recently damages brought by bacterial angular leaf spot to Chinese cabbage in north and
northeastern China has been aggravated year by year．Breeding cultivars resistant to angular leaf spot
has become an important research direction for breeding of Chinese cabbage．But until now a complete
identification method for Chinese cabbage resistance to angular leaf spot has not yet been established．
This experiment carried out isolation and identification on pathogen of bacterial angular leaf spot in Chinese
cabbage planted at the experiment and practice base of Northeast Agricultural University，and confirmed this
pathogen was Pseudomonas syringae pv. syringae van Hall．Taking this isolate as inoculating pathogen and
Chinese cabbage inbred line A224-1 as test material，this experiment adopted orthogonal experiment design of
L9（34），analyzed the effects of inoculation temperature，inoculation humidity and seedling age on occurrence
of bacterial angular leaf spot．The experiment also took inbred line 264-1-1，203-1 and 331-1 as test material
to screen out appropriate inoculating concentration．The results showed that the optimal condition combination
for occurrence of bacterial angular leaf spot in Chinese cabbage was 25-30 ℃ inoculation temperature 90%-
100% inoculation humidity 7-8 leaf-stage seedling age，and optimal inoculation concentration was 1 × 1010
cfu · mL-1．
2021 Vol. 1 (4): 64-68 [Abstract] ( 185 ) [HTML 1KB] [ PDF 1233KB] ( 605 )
69 Effects of Foliage Spraying of GeO2 on Germanium Accumulation and Nutritional Qualities of Garlic
QI Xiaofang，LI Xiaxia，LIU Zhongxiao，CHENG Zhihui
Germanium is one of the important functional components of garlic．Garlic is naturaly rich
in germanium and possesses higher health efficacy and medicinal value．However，depending on natural
germanium preservation store could cause problems as lower content and unstable levels．Taking garlic cultivar
‘G110’ as test material，this experiment sprayed different concentrations of GeO2 solutions（3，6，9，12 mg ·
L-1） by different times（once，twice，third times）；and studied the effects of exogenous germanium on garlic
germanium accumulation and nutrient quality．The results showed that the exogenous germanium concentration
extremely significantly affected the germanium content of garlic scape and bulb，and the spraying frequency
and their interactions only influenced the germanium content in garlic scape significantly．The Ge content in
garlic scape reached 27.00 μg · kg-1 when spraying 12 mg · L-1 GeO2 once on the leaf surface. Ge content was
increased by 66.9% comparing with the contrast（0 mg · L-1）．At the same time，the soluble protein content of
garlic scape was higher．Among all treatment combinations，the Ge content of bulb reached the highest level
of 4.02 μg · kg-1 by spraying 9 mg · L-1 GeO2 once．Therefore，9-12 mg · L-1 GeO2 solution can be sprayed on
garlic leaves during seedling establishment period for cultivation of Ge-rich garlic．
2021 Vol. 1 (4): 69-74 [Abstract] ( 189 ) [HTML 1KB] [ PDF 911KB] ( 409 )
75 Effect of Earthworm Activities on Growth，Yield of Continuous-cropping Lily and Fusarium Wilt Control
LU Yaoxiong，GAO Peng，LI Mianyong，CUI Xinwei，ZHU Xiaoqi，PENG Fuyuan，DAI Liangying
By pot experiments method，this paper studied the effect of earthworm activities on the
growth，yield，soil bulk density，rhizosphere microflora of continuous-cropping lily，and Fusarium
wilt control．The paper also analyzed the correlation between continuous-cropping lily Fusarium wilt and
rhizosphere soil biological characteristics．The results showed that the control effect of earthworm inoculation
on Fusarium wilt in continuous-cropping lily was obviously better than that of applying earthworm dung．
Comparing with the application of chemical fertilizer treatment，its wilt control effect was 53.42%，and lily
yield increases was 9.94%．The earthworm activities could increase soil moisture content，total porosity，
which are beneficial for seedling emerge ahead of schedule and growth，development of continuous-cropping
lily．Furthermore，earthworms activities had significantly enriched the count of bacterium and actinomycetes
in rhizosphere soil，while effectively brought down the number of fungus including Fusarium，thus reduced
the incidence rate and disease index of continuous-cropping lily wilt．To sum up，earthworm activities can
optimize the soil physical and chemical properties and microbial flora，reduce the occurrence of Fusarium wilt
in continuous cropping lily，and alleviate the obstacle for lily continuous-cropping．
2021 Vol. 1 (4): 75-81 [Abstract] ( 176 ) [HTML 1KB] [ PDF 1004KB] ( 502 )
82 Effects of Different Slow-release Fertilizers on Photosynthesis，Yield and Quality of Zizania aquatica
LI Tong，WU Zhanhui，TONG Jing，WANG Liping，WANG Baoju，LIU Ziying
In order to screen out the suitable slow-release fertilizer for Zizania aquatica, taking
Zizania aquatica‘ Zhejiao No.2’as test material and common compound fertilizer as the control，this paper
explored the effects of 5 different slow-release fertilizers with different ratios of nitrogen，phosphorus and
potassium on photosynthesis，yield and quality of Zizania aquatica．The results showed that by applying
‘Xinyangfeng’slow-release fertilizer with the ratio of nitrogen， phosphorus，potassium as 25∶12∶15，
the plant height of Zizania aquatica could reach 174.83 cm，total chlorophyll content be 5.56 mg · g-1，net
photosynthetic rate be 10.47 μmol · m-2 · s-1，yield be 27.44 t · hm-2，soluble protein content be 2.77 mg · g-1，
VC content be 110.90 mg · kg-1， and soluble sugar content be 3.62%．In conclusion，under the experimental
conditions，the botanical characters，photosynthetic parameters of leaves，yield and quality of Zizania
aquatica treated with‘Xinyangfeng’slow-release fertilizer were all better than those of the other treatments．
Thus，we recommended to extend and use this treatment in Zizania aquatica planting．
2021 Vol. 1 (4): 82-87 [Abstract] ( 175 ) [HTML 1KB] [ PDF 893KB] ( 544 )
88 Studies on Technology for Hybrid Seed Production of Long Eggplant in Southwest China
ZHOU Shanshan，TAO Tao，ZOU Min，YANG Yang，WANG Yongqing，TIAN Shibin
Taking ‘Yuqie No.5’ as test material and adopting orthogonal design experiment of 4 factors
and 3 levels L9（34），this paper studied the effects of different treatments on the yield of eggplant hybrid seed
production by comparing the seed yield，number of seed fruits，seed output per single fruit．The results
showed that among all treatments combined by 9 different factors，treatment 5（planting density 30 000
plants · hm-2），taking out the 1st and 2nd branches flowers，starting pollination from the 3rd branches，
emasculation in bud stage and re-pollination at flowering stage） obtained the highest yield of seed production
776.1 kg · hm-2．The results of range analysis showed that the factors influencing seed production
efficiency were pollination mode＞reserving commercial fruit＞starting branch of pollination＞planting
density．But，all these 4 factors had extreme significant effect on eggplant hybrid seed production．
Comprehensive comparing analysis indicated that the optimum seed production method was planting density
24 000 plants · hm-2，taking out the first and second branches flowers，starting pollination from the 3rd
branches，emasculation in bud stage and re-pollination at flowering stage，when conducting large-scale
eggplant hybrid seed production．
2021 Vol. 1 (4): 88-91 [Abstract] ( 180 ) [HTML 1KB] [ PDF 864KB] ( 629 )
92 A New Pepper F1 Hybrid —‘Xiangluo’
HE Jingwen，WU Di，FU Wenting，ZHU Yongpeng，CHEN Song
‘Xiangluo’is a new fresh pepper F1 hybrid developed by crossing inbred line‘10011A’as
female parent and inbred line‘jq55-1’as male parent．It is of medium maturity．Its growth period is about
170 days．The plant has strong growth vigor and successive fruit setting ability．Its plant height is 65 cm and
spread length is 65 cm．The fruit is of horn shape with wrinkled surface．The fruit is 16-20 cm in length and
1.3-1.8 cm in transverse diameter．The green ripe fruit is green in color，ripe one is bright red in color．The
VC content is 1 342.0 mg · kg-1，and capsaicin content is 0.12%．In fields，its resistance to virus disease is
stronger than that of the contrast‘Changla No.7’．The fresh pepper yield is about 33 t · hm-2．It is suitable for
spring planting in open fields of Guizhou regions．
2021 Vol. 1 (4): 92-94 [Abstract] ( 185 ) [HTML 1KB] [ PDF 2691KB] ( 458 )
95 A New Watermelon F1 Hybrid —‘Guifei’
XIE Huayun，HE Yi，HONG Rixin，YE Yunfeng，LI Guifen，QIN Sihua，HUANG Jinyan，LIU Tangjing，CHEN Dongkui，LI Tianyan
‘Guifei’is a new watermelon F1 hybrid of early maturity developed by crossing inbred
line‘HL4334’as female parent and inbred line‘HHH3-1’as male parent．In spring cultivation，its whole
growing period is about 96 days and the fruit development period is about 30 days．In autumn cultivation，its
whole growing period is about 75 days and the fruit development period is about 28 days．The plant has strong
growth vigor and is easy to bear fruit．Its fruit is of oval shape．The peel is dark green in color with dark green
stripes．The fruit peel is 0.8-1.0 cm in thickness．The flesh is bright red in color with delicate，sweat and crisp
quality．The center soluble solid content is 11.5%．The single fruit weight is 2.5-3.8 kg．It can yield about
34.5 t · hm-2．It is suitable for open field cultivation in Guangxi regions．
2021 Vol. 1 (4): 95-97 [Abstract] ( 179 ) [HTML 1KB] [ PDF 2681KB] ( 472 )
98 A New Bitter Gourd F1 Hybrid —‘Jintian 188’
XIN Yaning，LI Chunwen，LEI Yingcai，OUYANG Liying，WAN Qun，SHUN Bin，LIAN Huashan
‘Jintian 188’is a new early maturing bitter gourd F1 hybrid developed by crossing inbred
line‘CH-LS19·7’as female parent and inbred line‘GY-3QP12·8’as male parent．It takes about 90
days for spring greenhouse cultivation from sowing to harvest．The plant has strong growth vigor and the
germination ability of lateral branches at base is strong．Both the main stem and lateral stem can bear melon．
The first female flower sets on the 7-9th nodes，and female flowers appear continuously with 2-3 nodes
distance．The fruit is of long stick shape．The head is of shuttle shape and tail is pointed．The fruit peel is
green and white in color with alternating stripes and pearl nodules．The melon is 35-40 cm in length，6-7 cm
in diameter．Its pulp is 0.96-1.18 cm in thickness．The single fruit weight is 0.5-0.8 kg．This variety can
yield about 75 t · hm-2．Its fruit hardness is 12.97 kg · cm-2．It is tolerant to storage and transportation．The
shelf life is 4-5 days．In fields，its resistance to powdery mildew and downy mildew is stronger than that of
the control‘Fuxi 1918’．It is suitable for cultivation in most hilly areas and regions with similar ecological
conditions of Sichuan Province．
2021 Vol. 1 (4): 98-100 [Abstract] ( 143 ) [HTML 1KB] [ PDF 2683KB] ( 386 )
101 A New Sweet Corn F1 Hybrid —‘Shuangse 1424’
WANG Ying，WANG Xiaoling，XI Xiaofei，QIAO Zhihui，WANG Haixu，CHEN Fuzhi，ZHANG Zhengjie
‘Shuangse 1424’is a new fresh sweet corn F1 hybrid bred by crossing inbred line‘Huangtian
3148’as female parent and inbred line‘Baitian 2110’as male parent．Its growth period is about 90 days．
Its plant type is semi-compact．It is 209.5 cm in plant height and 77.5 cm in ear height．The ear is of post-box
type，21.0 cm in length and 5.1 cm in diameter．There are 15.3 head progeny rows and 37.3 grains per row．
The grain is yellow and white in color．The soluble sugar content is 10.0%．The amylopectin takes 98.5% of
the total starch content．It is moderate resistant to smut disease．The fresh ear yield is about 14.4 t · hm-2．It is
suitable to be planted in Hexi Corridor（Jiuquan，Zhangye，Wuwei），eastern regions（Lanzhou，Baiyin）of
Gansu Province and areas with similar ecological conditions．
2021 Vol. 1 (4): 101-103 [Abstract] ( 162 ) [HTML 1KB] [ PDF 2630KB] ( 343 )
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2021 Vol. 1 (4): 104-109 [Abstract] ( 177 ) [HTML 1KB] [ PDF 1554KB] ( 652 )
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2021 Vol. 1 (4): 109-111 [Abstract] ( 183 ) [HTML 1KB] [ PDF 999KB] ( 746 )
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2021 Vol. 1 (4): 112-114 [Abstract] ( 150 ) [HTML 1KB] [ PDF 920KB] ( 442 )
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2021 Vol. 1 (4): 115-117 [Abstract] ( 157 ) [HTML 1KB] [ PDF 1049KB] ( 396 )
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2021 Vol. 1 (4): 117-119 [Abstract] ( 177 ) [HTML 1KB] [ PDF 1577KB] ( 272 )
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2021 Vol. 1 (4): 120-122 [Abstract] ( 177 ) [HTML 1KB] [ PDF 1922KB] ( 791 )
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