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01 July 2021, Volume 1 Issue 7
    

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  • Research Progress on Genetic Breeding of Cucumber Tolerance for Salt Stress
    LIU Dongrang，DONG Shaoyun，MIAO Han，BO Kailiang，ZHANG Shengping，GU Xingfang
    China Vegetables. 2021, 1(7): 14-23.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Salt stress is one of the main abiotic stresses affecting cucumber growth and development．It
    is of important significance to carry out studies on cucumber tolerance for salt stress and cultivate salt tolerant
    varieties for improving cucumber salt tolerance and increasing use ratio of saline-alkali land．This paper
    reviewed the following 4 aspects：evaluation of salt tolerance in cucumber germplasm resources；effects of
    salt stress on cucumber growth，development and physiology；genetic analysis and genes mining related to
    cucumber salt tolerance and major measures for improving cucumber tolerance to salt stress，etc．Emphasis
    was laid on research progress made in cucumber breeding for tolerance to salt stress in recent years．At the
    same time，the paper put forward the problems existing in current research on cucumber breeding for tolerance
    to salt stress，and prospected the research directions in future．
  • Comparative Transcriptome Analysis of CMV Resistance of Pepper at Seedling Stage
    LEI Yang，CHENG Yan，QIAO Ning，JIAO Yansheng，WU Yueli
    China Vegetables. 2021, 1(7): 24-31.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In order to explore the molecular response mechanism of pepper under infection of cucumber
    mosaic virus and resistant genes related to this virus，the paper took susceptible material‘Qiemen’and
    resistant material‘JJ101’as objectives，and conducted high-throughput transcriptome sequencing
    on‘Qiemen’and‘JJ101’ before and after innoculation of CMV，and carried out studies on gene expression
    and function by bioinformatics method．The results showed that by using RPKM to calculate gene expression
    level，1 158 differentially expressed genes（DEGs）were screened out after ‘Qiemen’and‘JJ101’ were
    inoculated with CMV．Among them，643 DEGs were enriched in Gene Ontology（GO）．Their functions
    were mainly referred to enzyme activity，metabolic process and defense reaction，etc．While 287 DEGs could
    be included into KEGG pathways，including plant pathogen interaction，plant hormone signal transduction，
    etc. process．Studies discovered that pepper immunity to CMV was an extreme complicated and multi-pathway
    coordinated process，jointly participated by protein metabolism，defense response，hormone regulation and the
    other pathways，laying a theoretical foundation for further research on pepper resistant molecular mechanism．
  • Analysis of Differentially Expressed Genes of Sugar Metabolism in aga2 Mutant Watermelon Fruit under Low Temperature and Weak Light
    DING Shuangyu，REN Yi，GONG Guoyi，ZHANG Haiying，GUO Shaogui，YU Yongtao，LI Maoying，ZHANG Jie，XU Yong
    China Vegetables. 2021, 1(7): 32-39.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In order to explore the regulatory mechanism of low temperature and weak light environment
    on sugar accumulation of watermelon，this experiment analyzed the transcriptome data of mutant of alkaline
    alpha-galactosidase gene aga2，and found that there were 686 up-regulated genes and 990 down-regulated
    genes in aga2 mutant fruits under low temperature and weak light．By GO and KEGG analysis，it was further
    found that the up-regulated expression of transcription factors：MYB，NAC and WRKY etc. could resist low
    temperature and weak light．While，the down-regulated expression of genes related to sugar metabolism
    like galactinol synthase（Cla009222），alpha-galactosidase（Cla022883、Cla019238）etc. induced by
    low temperature and weak light may be the main reason for the decrease of sugar content in mutant fruit．
    By analyzing the transcriptome data，this experiment excavated the genes related to sugar accumulation in
    watermelon responding to low temperature and weak light，and laid the foundation for regulation network of
    sugar metabolism in watermelon responding to low temperature and weak light．
  • Effects of Different Topdressing Nitrogen Fertilizer on Quality，Growth，and Nitrogen Use Efficiency of Cherry Tomato in Solar Greenhouse
    LIU Yuxi，WANG Juanjuan，WU Longkai，LI Yansu，HE Chaoxing，YU Xianchang，WANG Jun
    China Vegetables. 2021, 1(7): 40-47.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Aiming at the issue of lacking rational nitrogen fertilizer parameter in producing cherry tomato
    under solar greenhouse，this paper carried out studies on the effects of different nitrogen fertilizer topdressing
    on the quality and nitrogen use efficiency in Beijing under moderate fertility soil conditions．In this study，
    cherry tomato‘Qianxi’and‘Hongyu’were selected as experimental materials．The normal amount of
    nitrogen fertilizer was determined according to target yield．Four treatments were set up，described as no
    nitrogen topdressing（CK），50% normal nitrogen topdressing（50%N），normal nitrogen opdressing（N），
    and 150% normal nitrogen topdressing（150%N），respectively．The results showed that compared with the
    CK，nitrogen topdressing could improve the yield and quality of cherry tomato；compared with N treatment，
    50%N treatment could increase the contents of soluble solid，soluble sugar，titratable acid，VC and nitrogen
    use efficiency in cherry tomato，but there were no significant influence on the plant growth，development and
    yield of cherry tomato．Therefore，under the condition of soil nutrients and organic fertilizer basal application
    closed to the experimental condition in this study，if taking cherry tomato‘Qianxi’ as cultivated variety and
    the target yield for winter-spring of‘Qianxi’ was 45 000 kg · hm-2，the recommended amount of fertilizer were
    N 120 kg · hm-2，P2O5 75 kg · hm-2，and K2O 360 kg · hm-2．If taking cherry tomato‘Hongyu’ as cultivated
    variety，the target yield for autumn-winter of‘Hongyu’ was 60 000 kg · hm-2，the recommended amount of
    fertilizer were N 135 kg · hm-2，P2O5 90 kg · hm-2，and K2O 405 kg · hm-2．
  • Effects of GABA and CaCl2 Compound on Growth，Chlorophyll Fluorescence Parameters and Reactive Oxygen of Tomato Seedlings under Salt and Alkali Stress
    CHEN Guangxin，DING Dong，LI Qian，CHEN Yingxue，WANG Yening，LYU Chenxi，GAO Hongbo，WU Xiaolei
    China Vegetables. 2021, 1(7): 48-55.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Gamma amino butyric acid（GABA）plays an important role in enhancing plants stress
    resistance．While，the synthesis of endogenous GABA is related to the concentration of intracellular Ca2+．
    Taking the salt sensitive tomato variety‘Zhongza No.9’as test material，and adopting salt alkali stress
    concentration（60 mmol · L-1 NaCl + 60 mmol · L-1 NaHCO3） with remarkable different phenotype，this
    experiment studied the effect of adding GABA and CaCl2 separately and adding their compound on growth，
    chlorophyll fluorescence and reactive oxygen，etc. indexes of tomato seedlings under salt and alkali stress．
    The results showed that the growth rates of plant height，stem diameter，root length and number of roots of
    tomato，its fresh weight and dry weight were all obviously increased under salt and alkali stress，no matter
    the exogenous GABA and CaCl2 were added solely or in combination．The leaf color index was significantly
    increased and chlorophyll fluorescence parameter was also obviously improved．At the same time，
    the exogenous GABA and CaCl2 separate treatment and compound treatment reduced the reactive oxygen
    accumulation of tomato leaves．Compared with the separate treatment，SA + CA6 + GABA5 treatment had a
    better alleviating effect on the injury by salt and alkali stress，indicating that the addition of exogenous CaCl2
    could promote the ability of GABA in enhancing plants salt and alkali tolerance．
  • Studies and Application of New Simplified Water-saving Cultivation Technology for Chinese Cabbage
    WU Ping，ZHANG Fenglan，YU Yangjun，WANG Weihong，WANG Dexin，SONG Shunhua，XU Xiulan，TANG Xiaowei，GUO Yu，ZHANG Haijun，WANG Baoju，GAO Yan
    China Vegetables. 2021, 1(7): 56-61.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In order to improve the simplified water-saving cultivation technology for Chinese cabbage
    〔Brassica campestris L. ssp. pekinensis（Lour）Olsson〕，this paper confirmed the application details of
    correlation technique implement．This study observed the effect of adding pesticide on seed germination and
    insect pests occurrence at the seedling stage，with different concentrations of phoxim and imidacloprid were
    added during the seed encapsulation process separately or simultaneously；investigated the effect of different
    seeding rate，different planting patterns on the emergence rate at seedling stage；and surveyed the planting
    benefits of different types of Chinese cabbage using precision seeding and simplified planting technology．The
    results showed that phoxim（effective ingredient content ≤ 3 g · kg-1）or imidacloprid（effective ingredient
    content ≤ 70 g · kg-1）added in the seed encapsulation could reduce the insect pests occurrence at the seedling
    stage without affecting seeds germination．The emergence rate of 2 seeds per hole could reach over 88%，and
    basically fulfilled the production needs．The emergence rate of drip irrigation was 95%-99%，significantly
    higher than that of the traditional furrow irrigation．Compared with the traditional cultivation pattern，
    water consumption and labor use were reduced by 10.4%-29.5% and 42.2%-79.0%，respectively by this new
    cultivation technology during Chinese cabbage growth period．
  • Glycoalkaloid Content in Tubers of Main Potato Cultivars in China and Effect of Mulching Methods and Processing on Its Glycoalkaloid Content
    XIONG Anping，CHEN Ming，LU Ying，QIN Yuzhi，PENG Zhong，XIONG Xingyao，HU Xinxi
    China Vegetables. 2021, 1(7): 62-68.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    High performance liquid chromatography-evaporative light scattering detection （HPLCELSD）
    method was used to measure the content of tuber glycoside alkaloids in main potato cultivars in China．
    This paper also studied the effects of different mulching methods and tuber processing on the content of potato
    glycoside alkaloids．The results showed that the glycoside alkaloid content of 51 main potato varieties in China
    was 106.28-3 770.50 mg · kg-1（DW）．Among them，there were 7 potato varieties whose glycoside alkaloid
    contents exceeded the safe value 1 000 mg · kg-1（DW）．Comparing with those unprocessed，the glycoside
    alkaloid content of potato tubers decreased significantly after baking，which was beneficial for improving the
    safety of potato tubers．The content of glycoside alkaloids in potato tubers covered with black plastic film was
    the lowest，which was significantly lower than that in open field cultivation．Soil mulching after plastic film
    mulching had little effect on tuber glycoside alkaloid content．So black plastic film mulching should be used to
    grow potato tubers，thus to obtain tubers with low glycoside alkaloid content．
  • Studies On Correlation Between Peel Hardness and Grain Plumpness of Seed-used Zucchini
    ZHU Hongyan，WEI Zhaoxin，ZHANG Yongjiu
    China Vegetables. 2021, 1(7): 69-73.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    This experiment measured peel hardness of 2 seed-used Cucurbita pepo sister lines by shore
    hardness tester，and carried out slice observation and grain plumpness measurement．The correlation between
    peel hardness and grain plumpness was also analyzed．The results showed that there were extremely significant
    differences in pericarp hardness of Cucurbita pepo sister lines．The peel hardness of Q212-6-3-1 with hard
    peel was 30.3 HD．The width of epidermis 10 columnar cells was 3 526.7 μm．The epicarp thickness was
    3 792.6 μm．The thickness of mid mesocarp was 27 700.0 μm．Its difference with soft peel Q212-6-3-2 was
    significant or extremely significant．The hundred-grain weight of hard peel fruit was extremely significant
    higher than that of the soft peel fruit．Pericarp hardness and hundred-grain weight presented extremely
    significant positive correlation．The harder of the peel，the bigger of the hundred-grain weight，the more
    plump of the grain．Therefore，hard pericarp could be taken as a major target trait for seed-used Cucurbita
    pepo     breeding．
  • Effects of Different Preceding Crops on Clubroot and Yield of Chinese Cabbage in Autumn
    LU Peng，LUO Xiaohan，WANG Xuedong，LIANG Xinyu，WU Fengzhi，PAN Kai
    China Vegetables. 2021, 1(7): 74-78.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    In order to screen out the suitable preceding crops for Chinese cabbage production in autumn in
    open fields of northern China，this paper took the long-term planting model at the locality of garlic + Chinese
    cabbage as the contrast；studied the effects of 3 different preceding crops including wheat，potato onion，and
    soybean on clubroot and yield of Chinese cabbage in autumn．The results showed that taking soybean，potato
    onion and wheat as preceding crops could reduce the content of resting spores of Plasmodiophora brassicae in
    spring-crop soil．The disease index of clubroot of autumn Chinese cabbage treated with potato onion + Chinese
    cabbage were all the time significantly lower than that of the contrast．The overground part fresh，dry weight
    and yield of Chinese cabbage in autumn treated with potato onion + Chinese cabbage and wheat + Chinese
    cabbage were all significantly higher than the overground part fresh，dry weight and yield of Chinese cabbage
    gained 30 days and 40 days after sowing．In summary，taking potato onion as the preceding crop can inhibit
    the occurance of clubroot in Chinese cabbage in autumn and increase its yield as well．This technology is
    suitable for application in local production．
  • Isolation and Identification of Fruit Rot Pathogen in Pumpkin
    LIU Wenjie，Wang Wenzheng，LUO Lan
    China Vegetables. 2021, 1(7): 79-82.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Post-harvest fruit rot has brought huge economic loss to pumpkin production．In order to
    exploit the pathogen of pumpkin fruit rot in Jiaozhou，Qingdao of Shandong Province，this study isolated and
    identified the pathogen by molecular biology and morphology method and according to Koch’s Rule．Results
    indicated that 2 kinds of bacterial strain were isolated from the infected pumpkin fruit．They were the pathogens
    causing pumpkin fruit rot．They were identified as Fusarium solani and Rhizopus stolonifer，respectively．
    This is the first report in China that Fusarium solani and Rhizopus stolonifer could infect pumpkin fruit．
  • Control Effect of Five Biological Pesticides Against Asparagus Stem Blight
    YANG Shuai，XU Shubing，JIN Yan，ZHANG Guofu，FENG Yongxin，WANG Hongyan
    China Vegetables. 2021, 1(7): 83-87.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    The bacteriostatic activity of 5 biological pesticides including Bacillus subtilis，lentinan，
    kasugamycin，polyoxin and amino-oligosaccharin against asparagus stem blight pathogen，plant safety
    and field control effect on these diseases were determined by growth rate method and stem leaf spray
    method．The results of growth rate test showed that lentinan and amino-oligosaccharin had no direct antibacterial
    activity against Phomopsis asparagi．The safety test showed that 1 200-2 400 t · hm-2 the Bacillus subtilis WP
    could inhibit the growth of asparagus．The results of two-year efficacy test showed that 7 days after the last
    application，the differences between the average control effect of high-dose of 5 biological pesticides and the
    contrast medicament difenoconazole were not significant．And they all showed better control effect on asparagus
    stem blight．Fourteen days after treatment，the field control effects of Bacillus subtilis WP，2% lentinan AS and 5%
    amino-oligosaccharides AS were all significantly higher than those of the contrast．The 5 biological pesticides
    could all be used for green prevention and control of asparagus stem blight，among them lentinan and aminooligosaccharides
    belong to plant inducers，had good control effect and longer effective duration．
  • Studies on Factors Affecting Quality and Safety of Flowering Chinese Cabbage Based on Delphi-AHP
    CHEN Huijun，LIU Huan，HUANG Zhuoquan，JIN Dengpeng，KE Qianhua，LIU Chunhong
    China Vegetables. 2021, 1(7): 88-92.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    Using Delphi-AHP method，this paper investigated and analyzed various factors affecting the
    quality and safety of flowering Chinese cabbage〔Brassica campestris L. ssp. chinensis（L.）var. utilis Tsen
    et Lee〕in the whole supply chain from farmland to dining table；and evaluated the importance of each factor
    in affecting the quality and safety of flowering Chinese cabbage．The results showed that the quality safety
    level during planting period among root base class had the maximum influence on quality of flowering Chinese
    cabbage．The top 5 factors affecting quality of flowering Chinese cabbage were production place environment
    and facilities qualified rate，disease and pest control level，pesticide and growth regulator residue percent
    of pass，preservatives residual percent of pass，storage environment and management standardization．The
    weighted were 0.130，0.130，0.130，0.128 and 0.082，respectively．
  • A New Cucumber F1 Hybrid —‘Longlyu No.3’
    XU Chunmei，ZHANG Zuobiao，LIU Jinglan，MENG Xuejiao，LIU Siyu，YE Wanjun，YAO Hongquan，YANG Long
    China Vegetables. 2021, 1(7): 93-95.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    ‘Longlyu No.3’is a new cucumber F1 hybrid developed by crossing inbred line‘JZ161’as
    female parent and inbred line‘4F’as male parent．Its plant has medium growth vigor．The fruits mainly
    grow on the main vine．The first female flower sets on the 3-5th nodes．The fruit is of straight and rodlike
    shape，and is about 30 cm in length with short stalk．Its peel is bright dark green in color with dense spines．
    The flesh is green in color．Its VC content is 130.0 mg · kg-1，and soluble solids content is 3.31%．The plant
    is resistant to downy mildew，powdery mildew，and blight．It can yield about 57 t · hm-2．It is suitable to be
    planted in the Three Northeast Provinces and Inner Mogolia in protected fields in early spring．
  • A New Chieh-qua F1 Hybrid —‘Jibao No.1’
    CHEN Muxi，XIE Yuebo，CHEN Kunhao，CHEN Ruzhu，DU Bingzi，ZHENG Hanfan
    China Vegetables. 2021, 1(7): 95-97.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    ‘Jibao No.1’is a new chieh-qua F1 hybrid developed by crossing inbred line‘Heimao’as
    female parent with inbred line‘Lyufeng’as male parent．It is of early-medium maturity．It takes 69 days in
    spring and 54 days in autumn from sowing to harvesting，and the harvest period can last for 36 days in spring
    and 33 days in autumn．Its fruit is of cylindrical shape．The peel is dark green in color with even distributed
    medium-sized white spots，but without furrows．The flesh is compact with superior quality．Its fruit is about
    19 cm in length，7.5 cm in transverse diameter，and 1.7 cm in flesh thickness．The single fruit weight is 600-
    720 g．The commodity fruit rate is about 95%．Its VC content is 219.0 mg · kg-1，soluble solid matter content
    is 36.0 mg · g-1，and crude protein content is 5.16 mg · g-1．It can yield 60.0-67.5 t · hm-2．It is suitable for
    autumn cultivation in chieh-qua producing regions of Guangdong Province．
  • A New Chinese Chives Variety —‘Pengbao’
    LI Jijun，LI Yixiao，MA Peifang，LI Yunying，LYU Aiqin，YIN Shouheng，CHEN Jianhua
    China Vegetables. 2021, 1(7): 98-100.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    ‘Pengbao’is a new Chinese chives variety of developed by crossing a favor
    strain‘Guilindaye’as female parent with an inbred line‘Jinyin No.1’as male parent through sexual
    hybridization and systematic breeding．It is of erect plant type and about 49 cm in plant height．Its leaf
    blades are green in color with 40 cm in length，and 0.7-1.1 cm in width．The single plant has 4-6 leaves．
    The average single plant weight is 9.6 g．It has strong growth vigor．It is strongly resistant to cold and will
    not go dormant in winter．In fields，its resistance to grey mold is equivalent to that of the contrast‘791’．
    Its VC content is 302 mg · kg-1，and β-carotene content is 14.70 μg · g-1．It can be harvested 6-7 times a year
    and yield about 195 t · hm-2．It is suitable to be cultivated in open and protected fields in Henan Province and
    surrounding areas with similar ecological conditions．
  • A New Fresh Edible Sweet and Waxy Corn F1 Hybrid —‘Baitiannuo 209’
    WANG Jun，LI Hongtao，XU Hanyuan，ZHU Qing，LI Shufen，CHI Ming
    China Vegetables. 2021, 1(7): 101-103.
    Abstract ( ) Download PDF ( )   Knowledge map   Save
    ‘Baitiannuo 209’is a new fresh edible sweet and waxy corn F1 hybrid bred by crossing inbred
    line‘LN38’as female parent and inbred line‘LN205’as male parent．It is of medium-early maturity．
    It takes about 83 days from sowing to fresh ear harvesting．It is of semi-compact plant type．It is 217 cm in
    plant height and 105 cm in ear position height．Its ear is of cylindrical shape，19.1 cm in length and 4.5 cm
    in ear diameter．There are 14 rows in each ear and 33 grains in one line．Its grain is white in color．The
    proportion of sweet and waxy grain is 1∶3．The amylopectin content takes 97.5% of the total starch content．
    The hide trimmings rate is 8.1%．It can yield fresh ear about 13.5 t · hm-2．It is resistant to large spot disease
    and medium resistant to small spot disease and sheath blight．It is suitable to be planted in Jiangsu Province and
    surrounding areas with same ecological conditions．
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