FU Kejian，ZHOU Hongyin，ZHANG Naiming，ZHANG Jilai，DENG Yishu，HANG Jiakui，FENG Yuxing，SU Youbo
Using Chinese cabbage〔Brassica campestris L. ssp. pekinensis（Lour）Olsson〕‘Hanyukuaicai’as the test material and employing the potted planting method，this study investigated the efficacy of different concentrations of wood vinegar solution（diluted 600，400，and 200 times）in controlling clubroot disease caused by Plasmodiophora brassicae．The Hochest33342-PI dual fluorescence staining method was employed to determine the spore viability of Plasmodiophora brassicae under different concentrations of wood vinegar treatment．Additionally，the main antimicrobial components of wood vinegar were analyzed．The results showed that all three concentrations of wood vinegar treatment significantly increased the plant height and aboveground fresh weight of Chinese cabbage．They also significantly reduced the incidence and disease index of clubroot disease，with a significant decreasing trend observed with increasing wood vinegar concentration．Specifically，the incidence of clubroot disease in Chinese cabbage treated with wood vinegar diluted 200 times was only 14.42%，achieving a control efficiency of 68.72%．Wood vinegar could deactivate the spores of Plasmodiophora brassicae，with a spore survival rate of only 24.03% under treatment with wood vinegar diluted 200 times．Using GC-MS technology，24 components were preliminarily determined to be present in the wood vinegar tested，among which 17 components exhibited certain inhibitory effects on Plasmodiophora brassicae spores．Among them，the inhibitory effect of shu-chuang-mu-phenol was the strongest，with an EC50 of 113.01 μg · mL-1．In conclusion，under the conditions of this experiment，wood vinegar diluted 200 times was found to be the most effective for controlling clubroot disease in Chinese cabbage．
